人工和天然牙釉质龋损表层的有机物含量及渗透性变化

Organic volume and permeability variations in the surface layer of artificial and natural enamel carious lesions.

作者信息

de Holanda Ferreira Danilo Augusto, Rolim de Abreu Nathalie Murielly, Meira Kássia Regina Simões, de Sousa Frederico Barbosa

机构信息

Graduate Program in Dentistry, Health Sciences Center, Federal University of Paraiba, Cidade Universitária, 58051-900 João Pessoa, Paraiba, Brazil.

Graduate Program in Dentistry, Health Sciences Center, Federal University of Paraiba, Cidade Universitária, 58051-900 João Pessoa, Paraiba, Brazil; Department of Morphology, Health Science Center, Federal University of Paraiba, Cidade Universitária, 58051-900 João Pessoa, Paraiba, Brazil.

出版信息

Arch Oral Biol. 2023 Apr;148:105645. doi: 10.1016/j.archoralbio.2023.105645. Epub 2023 Feb 12.

DOI:10.1016/j.archoralbio.2023.105645

PMID:36804643

Abstract

OBJECTIVES

Test the hypothesis the type of enamel caries (natural, artificial induced by gel, and artificial induced by acid solutions) affect the organic volume and the permeability of the surface layer in enamel caries lesions.

DESIGN

Artificial enamel caries, induced by either acidic solution (organic-poor; Group 1) or acidic gel (organic-rich; Group 2), and natural non-cavitated inactive approximal enamel caries lesions (NEC; Group 3) were obtained, from which longitudinal ground sections were prepared. Measurements of the mineral (V) (by microradiography), and water (α) and organic (β) volumes (by optical birefringence) were obtained at three points in the surface layer (n = 30/group).

RESULTS

The main outcomes were the ratio between experimental β by predicted β (β Ratio) and the ratio between experimental and predicted permeabilities (α Ratio). β Ratio in Group 1 was lower than in Groups 2 (Cohen's d: -1.81; 95% CI:-1.45,-2.32; p < 0.001) and 3 (Cohen's d: -0.71; 95% CI:-0.27,-1.18; p = 0.004), and Group 2 surpassed Group 3 (Cohen's d: 0.49; 95% CI:0.07,0.94; p = 0.03). α Ratio in Group 1 was higher than in Groups 2 (Cohen's d: 1.86; 95% CI:1.49,2.33; p < 0.001) and 3 (Cohen's d: 0.60; 95% CI:0.18,1.14; p = 0.01), and Group 3 surpassed Group 2 (Cohen's d: 0.61; 95% CI:0.23,1.07; p = 0.01).

CONCLUSIONS

The highest organic volume and the lowest permeability occurred at the surface layer of gel-induced artificial enamel caries lesions, which should be preferred in in vitro studies on de- and remineralization and resin infiltration.

摘要

目的

验证以下假设，即釉质龋的类型（自然龋、凝胶诱导的人工龋和酸溶液诱导的人工龋）会影响釉质龋损表层的有机物含量和渗透性。

设计

制备了酸性溶液诱导（低有机物含量；第1组）、酸性凝胶诱导（高有机物含量；第2组）的人工釉质龋，以及自然的非龋性静止邻面釉质龋损（NEC；第3组），并从这些样本中制备纵向磨片。通过显微放射摄影测量表层三个点的矿物质含量（V），通过光学双折射测量水含量（α）和有机物含量（β）（每组n = 30）。

结果

主要结果为实验β与预测β的比值（β比值）以及实验渗透性与预测渗透性的比值（α比值）。第1组的β比值低于第2组（Cohen's d：-1.81；95% CI：-1.45，-2.32；p < 0.001）和第3组（Cohen's d：-0.71；95% CI：-0.27，-1.18；p = 0.004），第2组超过第3组（Cohen's d：0.49；95% CI：0.07，0.94；p = 0.03）。第1组的α比值高于第2组（Cohen's d：1.86；95% CI：1.49，2.33；p < 0.001）和第3组（Cohen's d：0.60；95% CI：0.18，1.14；p = 0.01），第3组超过第2组（Cohen's d：0.61；95% CI：0.23，1.07；p = 0.01）。