Wu Houkai, Huang Sijie, Wang Kaijun, Liu Zhidan
Laboratory of Environment-Enhancing Energy (E2E), College of Water Resources and Civil Engineering, China Agricultural University, Beijing 100083, China; Key Laboratory of Agricultural Engineering in Structure and Environment, Ministry of Agriculture, Beijing 100083, China; State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China.
Laboratory of Environment-Enhancing Energy (E2E), College of Water Resources and Civil Engineering, China Agricultural University, Beijing 100083, China; Key Laboratory of Agricultural Engineering in Structure and Environment, Ministry of Agriculture, Beijing 100083, China; Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agriculture Sciences, Beijing 100081, China.
Sci Total Environ. 2023 May 10;872:162238. doi: 10.1016/j.scitotenv.2023.162238. Epub 2023 Feb 15.
In search of the candidate for animal feed and clean energy, a new vision of algal biorefinery was firstly proposed to coproduce amino acids and biohythane via hydrothermal treatment and two-stage anaerobic fermentation. This study focused on the comprehensive analysis of amino acids recovered from Chlorella sp. and the subsequent biohythane production from microalgal residues. The content and recovery rate of amino acids were in the range of 2.07-27.62 g/100 g and 3.65 %-48.66 % with increasing temperature due to more cell wall disruptions. Furthermore, it was rich in essential amino acids for livestock, including leucine, arginine, isoleucine, valine and phenylalanine. A comparable hydrogen production (9 mL/g volatile solids (VS)) was reached at 70 °C and 90 °C, while it reduced to 5.84 mL/gVS at 150 °C. The group at 70 °C got the maximum methane generation of 311.9 mL/gVS, which was 16.67 %, 24.94 %, 38.38 % and 46.49 % higher than that of other groups. Microalgal residues at lower temperature contained more organics, which was the reason for the better biohythane production. The coproduction of amino acids and biohythane at 130 °C was favorable, which led to 43.71 % amino acids recovery and 93.82 mL biohythane production from per gVS of Chlorella sp. The improved microalgal biorefinery could provide an alternative way to mitigate the crisis of food and energy, but animal experimentations and techno-economic assessments should be considered for further study.
为寻找动物饲料和清洁能源的候选产品，首次提出了一种新的藻类生物精炼愿景，即通过水热处理和两段式厌氧发酵联产氨基酸和生物氢气。本研究重点对小球藻中回收的氨基酸进行综合分析，并对微藻残渣后续生产生物氢气进行研究。随着温度升高，由于细胞壁破裂增多，氨基酸含量和回收率分别在2.07 - 27.62 g/100 g和3.65% - 48.66%范围内。此外，它富含家畜必需氨基酸，包括亮氨酸、精氨酸、异亮氨酸、缬氨酸和苯丙氨酸。在70℃和90℃时产氢量相当（9 mL/g挥发性固体（VS）），而在150℃时降至5.84 mL/gVS。70℃组的甲烷最大产量为311.9 mL/gVS，比其他组分别高16.67%、24.94%、38.38%和46.49%。较低温度下的微藻残渣含有更多有机物，这是生物氢气产量更高的原因。在130℃时联产氨基酸和生物氢气效果良好，每克小球藻VS可实现43.71%的氨基酸回收率和93.82 mL生物氢气产量。改进后的微藻生物精炼可为缓解食品和能源危机提供一种替代途径，但进一步研究应考虑动物实验和技术经济评估。
