Atsu Prince M, Thompson Gary L
Department of Chemical Engineering, Rowan University, Glassboro, NJ 08028, United States of America.
Biomed Phys Eng Express. 2023 Feb 28;9(2). doi: 10.1088/2057-1976/acbd54.
Electropermeabilization of biomembranes often is measured by microscopic imaging of a membrane-impermeable fluorophore that penetrates the cells following pulsed electric field (PEF) exposure. PEF exposure subsequently changes physiological properties of tissue. One way to probe these changes in tissue is measuring electrical properties by way of electrochemical impedance spectroscopy (EIS). In this study, we analyse impedance and conductivity of bovine annulus fibrosus (AF) cartilage before and after exposures to PEF of 100s duration. Two PEF parameters-electric field amplitude and number of pulses-are varied, and total specific dose of PEF is calculated. AF tissue conductivity increases with both amplitude and number of pulses, indicating electropermeabilization of the AF cells. A Live/Dead cell imaging assay validates the EIS measurements, indicating intratissue cell permeabilization bysPEF exposure. These results support the extension of EIS to monitor extent of electropermeabilization of cells within cartilage tissue.
生物膜的电通透化通常通过对一种膜不透性荧光团进行显微成像来测量，该荧光团在脉冲电场（PEF）暴露后穿透细胞。PEF暴露随后会改变组织的生理特性。探测组织中这些变化的一种方法是通过电化学阻抗谱（EIS）测量电特性。在本研究中，我们分析了牛纤维环（AF）软骨在暴露于持续100秒的PEF前后的阻抗和电导率。改变了两个PEF参数——电场幅度和脉冲数，并计算了PEF的总比剂量。AF组织电导率随幅度和脉冲数增加，表明AF细胞发生了电通透化。活/死细胞成像分析验证了EIS测量结果，表明sPEF暴露使组织内细胞发生了通透化。这些结果支持将EIS扩展用于监测软骨组织内细胞的电通透化程度。
