Atsu Prince M, Mowen Connor, Thompson Gary L
Department of Chemical Engineering, Rowan University, Glassboro, New Jersey 08028, United States.
Department of Biomedical Engineering, Rowan University, Glassboro, New Jersey 08028, United States.
ACS Omega. 2023 Sep 30;8(40):36815-36822. doi: 10.1021/acsomega.3c03518. eCollection 2023 Oct 10.
This study is the first to report the enhancement of cell migration and proliferation induced by in vitro microsecond pulsed electric field (μsPEF) exposure of primary bovine annulus fibrosus (AF) fibroblast-like cells. AF primary cells isolated from fresh bovine intervertebral disks (IVDs) are exposed to 10 and 100 μsPEFs with different numbers of pulses and applied electric field strengths. The results indicate that 10 μs-duration pulses induce reversible electroporation, while 100 μs pulses induce irreversible electroporation of the cells. Additionally, μsPEF exposure increased AF cell proliferation up to 150% while increasing the average migration speed by 0.08 μm/min over 24 h. The findings suggest that the effects of PEF exposure on cells are multifactorial-depending on the duration, intensity, and number of pulses used in the stimulation. This highlights the importance of optimizing the μsPEF parameters for specific cell types and applications. For instance, if the goal is to induce cell death for cancer treatment, then high numbers of pulses can be used to maximize the lethal effects. On the other hand, if the goal is to enhance cell proliferation, a combination of the number of pulses and the applied electric field strength can be tuned to achieve the desired outcome. The information gleaned from this study can be applied in the future to in vitro cell culture expansion and tissue regeneration.
本研究首次报道了体外微秒级脉冲电场（μsPEF）暴露对原代牛纤维环（AF）成纤维样细胞迁移和增殖的促进作用。从新鲜牛椎间盘（IVD）分离的AF原代细胞暴露于不同脉冲数和施加电场强度的10 μs和100 μs PEF。结果表明，10 μs持续时间的脉冲诱导可逆电穿孔，而100 μs脉冲诱导细胞不可逆电穿孔。此外，μsPEF暴露使AF细胞增殖增加高达150%，同时在24小时内平均迁移速度提高0.08 μm/min。研究结果表明，PEF暴露对细胞的影响是多因素的，取决于刺激中使用的脉冲持续时间、强度和数量。这突出了针对特定细胞类型和应用优化μsPEF参数的重要性。例如，如果目标是诱导细胞死亡用于癌症治疗，那么可以使用大量脉冲来最大化致死效应。另一方面，如果目标是增强细胞增殖，可以调整脉冲数和施加电场强度的组合以实现预期结果。从本研究中收集的信息未来可应用于体外细胞培养扩增和组织再生。
