Zhao Yiyan, Liu Weimin, Zhao Xiaoming, Yu Zhitao, Guo Hongfang, Yang Yang, Moussian Bernard, Zhu Kun Yan, Zhang Jianzhen
Research Institute of Applied Biology, Shanxi Key Laboratory of Nucleic Acid Biopesticides, The Key Laboratory of Chemical Biology and Molecular, Engineering of Ministry of Education, Shanxi University, Taiyuan, Shanxi 030006, China; College of Life Science, Shanxi University, Taiyuan, China.
Research Institute of Applied Biology, Shanxi Key Laboratory of Nucleic Acid Biopesticides, The Key Laboratory of Chemical Biology and Molecular, Engineering of Ministry of Education, Shanxi University, Taiyuan, Shanxi 030006, China.
Int J Biol Macromol. 2023 May 1;236:123746. doi: 10.1016/j.ijbiomac.2023.123746. Epub 2023 Feb 17.
Lipophorin is the most abundant lipoprotein particle in insect hemolymph. Lipophorin receptor (LPR) is a glycoprotein that binds to the lipophorin and mediates cellular uptake and metabolism of lipids by endocytosis. However, the roles of LPR in uptake of lipids in the integument and ovary remain unknown in the migratory locust (Locusta migratoria). In present study, we characterized the molecular properties and biological roles of LmLPR in L. migratoria. The LmLPR transcript level was high in the first 2 days of the adults after eclosion, then gradually declined. LmLPR was predominately expressed in fat body, ovary and integument. Using immuno-detection methods, we revealed that LmLPR was mainly localized in the membrane of oenocytes, epidermal cells, fat body cells and follicular cells. RNAi-mediated silencing of LmLPR led to a slight decrease of the cuticle hydrocarbon contents but with little effect on the cuticular permeability. However, the neutral lipid content was significantly decreased in the ovary after RNAi against LmLPR, which led to a retarded ovarian development. Taken together, our results indicated that LmLPR is involved in the uptake and accumulation of lipids in the ovary and plays a crucial role in ovarian development in L. migratoria. Therefore, LmLPR could be a promising RNAi target for insect pest management by disrupting insect ovarian development.
脂磷蛋白是昆虫血淋巴中含量最丰富的脂蛋白颗粒。脂磷蛋白受体（LPR）是一种糖蛋白，它与脂磷蛋白结合，并通过内吞作用介导细胞对脂质的摄取和代谢。然而，在飞蝗（Locusta migratoria）中，LPR在体壁和卵巢脂质摄取中的作用尚不清楚。在本研究中，我们对飞蝗中LmLPR的分子特性和生物学作用进行了表征。LmLPR转录水平在成虫羽化后的头两天较高，然后逐渐下降。LmLPR主要在脂肪体、卵巢和体壁中表达。使用免疫检测方法，我们发现LmLPR主要定位于oenocytes、表皮细胞、脂肪体细胞和滤泡细胞的膜上。RNA干扰介导的LmLPR沉默导致表皮碳氢化合物含量略有下降，但对表皮通透性影响不大。然而，针对LmLPR进行RNA干扰后，卵巢中的中性脂质含量显著下降，这导致卵巢发育迟缓。综上所述，我们的结果表明LmLPR参与了卵巢中脂质的摄取和积累，并在飞蝗的卵巢发育中起关键作用。因此，LmLPR可能是通过破坏昆虫卵巢发育来进行害虫治理的一个有前景的RNA干扰靶点。
