Graduate School of Life Science, Transdisciplinary Life Science Course, Hokkaido University, Sapporo, Japan.
Department of Mathematics, Comilla University, Cumilla, Bangladesh.
FEBS Lett. 2023 Jun;597(11):1517-1527. doi: 10.1002/1873-3468.14600. Epub 2023 Feb 28.
An essential challenge in diagnosing states of nonalcoholic fatty liver disease (NAFLD) is the early prediction of progression from nonalcoholic fatty liver (NAFL) to nonalcoholic steatohepatitis (NASH) before the disease progresses. Histological diagnoses of NAFLD rely on the appearance of anomalous tissue morphologies, and it is difficult to segment the biomolecular environment of the tissue through a conventional histopathological approach. Here, we show that hyperspectral Raman imaging provides diagnostic information on NAFLD in rats, as spectral changes among disease states can be detected before histological characteristics emerge. Our results demonstrate that Raman imaging of NAFLD can be a useful tool for histopathologists, offering biomolecular distinctions among tissue states that cannot be observed through standard histopathological means.
诊断非酒精性脂肪性肝病 (NAFLD) 状态的一个重要挑战是在疾病进展之前，尽早预测从非酒精性脂肪肝 (NAFL) 向非酒精性脂肪性肝炎 (NASH) 的进展。NAFLD 的组织学诊断依赖于异常组织形态的出现，并且通过常规组织病理学方法很难对组织的生物分子环境进行分割。在这里，我们表明，高光谱拉曼成像为大鼠的 NAFLD 提供了诊断信息，因为在组织学特征出现之前，可以检测到疾病状态之间的光谱变化。我们的结果表明，NAFLD 的拉曼成像可以成为组织病理学家的有用工具，为无法通过标准组织病理学方法观察到的组织状态提供生物分子区分。
