Brantmeier S A, Bellin M E, Boehm S K, Bushmeyer S M, Kubajak C L, Dentine M R, Grummer R R, Ax R L
Department of Dairy Science, University of Wisconsin, Madison 53706.
J Dairy Sci. 1987 Oct;70(10):2138-44. doi: 10.3168/jds.S0022-0302(87)80265-5.
Concentrations of estradiol-17 beta in follicular fluid were correlated to follicular size, stage of estrous cycle, location of corpus luteum, and presence of large follicles. Paired ovaries were obtained from 481 nonpregnant cows at slaughter and follicles were classified as ipsilateral or contralateral to the corpus luteum. Follicular fluid estradiol-17 beta concentrations from 2494 small, 1485 medium, and 396 large follicles were quantified by radioimmunoassay. Stage of estrous cycle was estimated by visual examination of the corpus luteum. Follicles in stage 1 of the estrous cycle (d 1 to 4) had the highest estradiol-17 beta concentration and the smallest mean follicular diameter. Location of follicles relative to the corpus luteum had no influence on estradiol-17 beta concentrations. As follicular size increased, concentration of estradiol-17 beta also increased. The presence of a single large follicle did not affect the concentration of estradiol-17 beta in medium or small follicles. In contrast, if multiple large follicles occurred in the same cow, concentrations of estradiol-17 beta were significantly lower in medium but not small follicles.
卵泡液中雌二醇-17β的浓度与卵泡大小、发情周期阶段、黄体位置以及大卵泡的存在情况相关。从481头屠宰时未怀孕的母牛获取成对的卵巢，并将卵泡分类为与黄体同侧或对侧。通过放射免疫分析法对2494个小卵泡、1485个中等卵泡和396个大卵泡的卵泡液雌二醇-17β浓度进行了定量。通过肉眼检查黄体来估计发情周期阶段。发情周期第1阶段（第1天至第4天）的卵泡雌二醇-17β浓度最高，平均卵泡直径最小。卵泡相对于黄体的位置对雌二醇-17β浓度没有影响。随着卵泡大小的增加，雌二醇-17β的浓度也增加。单个大卵泡的存在并不影响中等或小卵泡中雌二醇-17β的浓度。相比之下，如果同一头母牛出现多个大卵泡，中等卵泡而非小卵泡中的雌二醇-17β浓度会显著降低。
