Brantmeier S A, Grummer R R, Ax R L
Department of Dairy Science, University of Wisconsin, Madison 53706.
J Dairy Sci. 1987 Oct;70(10):2145-9. doi: 10.3168/jds.S0022-0302(87)80266-7.
This study compared lipoprotein phospholipid, cholesterol, protein, and glycosaminoglycans in the lipoprotein fraction among follicular fluid pools derived from 2633 small (less than 6 mm), 1702 medium (6 to 10 mm), and 491 large (11 to 20 mm) bovine follicles subdivided by estradiol-17 beta concentrations. High density lipoproteins were the only lipoprotein class found in follicular fluid when apolipoprotein profile of the less than 1.21 g/ml density fraction was analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis and silver staining. Within a follicular size class, concentrations of lipoprotein constituents did not change among follicular fluid pools with varying estradiol-17 beta concentrations. As follicular size increased, follicular fluid lipoprotein phospholipid, cholesterol, and protein significantly increased. The percentage of total follicular fluid glycosaminoglycans in the less than 1.21 g/ml density fraction was highest for small follicles but averaged less than 5% for all follicular size classes. Therefore, bovine follicular fluid contains only high density lipoproteins and concentrations increased with follicular maturation. That increase in high density lipoprotein could be a potentially important source of cholesterol for progesterone production after the LH surge.
本研究比较了2633个小（小于6毫米）、1702个中（6至10毫米）和491个大（11至20毫米）的牛卵泡液池中脂蛋白部分的脂蛋白磷脂、胆固醇、蛋白质和糖胺聚糖，这些卵泡根据雌二醇-17β浓度进行了细分。当使用十二烷基硫酸钠聚丙烯酰胺凝胶电泳和银染分析密度小于1.21 g/ml部分的载脂蛋白谱时，高密度脂蛋白是卵泡液中唯一发现的脂蛋白类别。在一个卵泡大小类别内，不同雌二醇-17β浓度的卵泡液池之间脂蛋白成分的浓度没有变化。随着卵泡大小增加，卵泡液脂蛋白磷脂、胆固醇和蛋白质显著增加。小于1.21 g/ml密度部分的总卵泡液糖胺聚糖百分比在小卵泡中最高，但在所有卵泡大小类别中平均不到5%。因此，牛卵泡液仅含有高密度脂蛋白，且其浓度随着卵泡成熟而增加。高密度脂蛋白的增加可能是促黄体生成素激增后孕酮产生的胆固醇的潜在重要来源。
