Department of Clinical Sciences, College of Veterinary Medicine, Ohio State University, Columbus, Ohio, USA.
J Vet Intern Med. 2023 Mar;37(2):556-566. doi: 10.1111/jvim.16656. Epub 2023 Feb 17.
Hypoxia is a key driver of fibrosis and is associated with capillary rarefaction in humans.
Characterize capillary rarefaction in cats with chronic kidney disease (CKD).
Archival kidney tissue from 58 cats with CKD, 20 unaffected cats.
Cross-sectional study of paraffin-embedded kidney tissue utilizing CD31 immunohistochemistry to highlight vascular structures. Consecutive high-power fields from the cortex (10) and corticomedullary junction (5) were digitally photographed. An observer counted and colored the capillary area. Image analysis was used to determine the capillary number, average capillary size, and average percent capillary area in the cortex and corticomedullary junction. Histologic scoring was performed by a pathologist masked to clinical data.
Percent capillary area (cortex) was significantly lower in CKD (median 3.2, range, 0.8-5.6) compared to unaffected cats (4.4, 1.8-7.0; P = <.001) and was negatively correlated with serum creatinine concentrations (r = -.36, P = .0013), glomerulosclerosis (r = -0.39, P = <.001), inflammation (r = -.30, P = .009), and fibrosis (r = -.30, P = .007). Capillary size (cortex) was significantly lower in CKD cats (2591 pixels, 1184-7289) compared to unaffected cats (4523 pixels, 1801-7618; P = <.001) and was negatively correlated with serum creatinine concentrations (r = -.40, P = <.001), glomerulosclerosis (r = -.44, P < .001), inflammation (r = -.42, P = <.001), and fibrosis (r = -.38, P = <.001).
Capillary rarefaction (decrease in capillary size and percent capillary area) is present in kidneys of cats with CKD and is positively correlated with renal dysfunction and histopathologic lesions.
缺氧是纤维化的关键驱动因素，并与人类毛细血管稀疏有关。
描述患有慢性肾脏病（CKD）的猫的毛细血管稀疏情况。
58 只患有 CKD 的猫和 20 只未受影响的猫的存档肾脏组织。
对石蜡包埋的肾脏组织进行横断面研究，利用 CD31 免疫组织化学来突出血管结构。从皮质（10 个）和皮质髓质交界处（5 个）连续拍摄高倍视野的数字照片。观察者对毛细血管区域进行计数和着色。图像分析用于确定皮质和皮质髓质交界处的毛细血管数量、平均毛细血管大小和平均毛细血管面积百分比。病理学家对临床数据进行了盲法评估，并进行组织学评分。
与未受影响的猫（4.4，1.8-7.0）相比，CKD 猫的毛细血管面积百分比（皮质）明显降低（中位数 3.2，范围 0.8-5.6；P<.001），并且与血清肌酐浓度呈负相关（r = -.36，P =.0013），肾小球硬化（r = -.39，P =.001），炎症（r = -.30，P =.009）和纤维化（r = -.30，P =.007）。CKD 猫的毛细血管大小（皮质）明显低于未受影响的猫（2591 像素，1184-7289）（P<.001），并且与血清肌酐浓度呈负相关（r = -.40，P<.001），肾小球硬化（r = -.44，P<.001），炎症（r = -.42，P =.001）和纤维化（r = -.38，P =.001）。
患有 CKD 的猫的肾脏存在毛细血管稀疏（毛细血管大小和毛细血管面积百分比降低），并且与肾功能障碍和组织病理学病变呈正相关。
