Engineering Research Center of Soil Remediation of Fujian Province University; College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling, Shaanxi 712100, China.
J Exp Bot. 2023 Apr 18;74(8):2740-2753. doi: 10.1093/jxb/erad066.
The root-associated microbiomes play important roles in plant growth. However, it is largely unknown how wheat variety evolutionary relatedness shapes each subcommunity in the root microbiome and, in turn, how these microbes affect wheat yield and quality. Here we studied the prokaryotic communities associated with the rhizosphere and root endosphere in 95 wheat varieties at regreening and heading stages. The results indicated that the less diverse but abundant core prokaryotic taxa occurred among all varieties. Among these core taxa, we identified 49 and 108 heritable amplicon sequence variants, whose variations in relative abundances across the root endosphere and rhizosphere samples were significantly affected by wheat variety. The significant correlations between phylogenetic distance of wheat varieties and prokaryotic community dissimilarity were only observed in non-core and abundant subcommunities in the endosphere samples. Again, wheat yield was only significantly associated with root endosphere microbiota at the heading stage. Additionally, wheat yield could be predicted using the total abundance of 94 prokaryotic taxa as an indicator. Our results demonstrated that the prokaryotic communities in the root endosphere had higher correlations with wheat yield and quality than those in the rhizosphere; thus, managing root endosphere microbiota, especially core taxa, through agronomic practices and crop breeding, is important for promoting wheat yield and quality.
根相关微生物组在植物生长中发挥着重要作用。然而，小麦品种进化亲缘关系如何塑造根微生物组的每个亚群，以及这些微生物如何影响小麦的产量和品质，在很大程度上还不清楚。在这里，我们在返青和抽穗期研究了 95 个小麦品种的根际和根内细菌群落。结果表明，所有品种中都存在多样性较低但丰度较高的核心细菌分类群。在这些核心分类群中，我们鉴定出了 49 个和 108 个可遗传的扩增子序列变异体，它们在根内和根际样本中的相对丰度变化受到小麦品种的显著影响。在非核心和丰富的内根际样本亚群中，仅观察到小麦品种的系统发育距离与细菌群落相似性之间的显著相关性。同样，在抽穗期，只有根内微生物群与小麦产量显著相关。此外，还可以使用 94 个原核分类群的总丰度作为指标来预测小麦产量。我们的研究结果表明，根内微生物群与小麦的产量和品质的相关性高于根际微生物群；因此，通过农业实践和作物育种来管理根内微生物群，特别是核心分类群，对提高小麦的产量和品质很重要。
