Leibniz Institute of Photonic Technology (Leibniz-IPHT), Albert-Einstein-Street 9, 07745, Jena, Germany.
Institute of Physical Chemistry, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany.
Chemistry. 2023 Apr 25;29(24):e202300224. doi: 10.1002/chem.202300224. Epub 2023 Mar 24.
BODIPY-based donor-acceptor dyads are widely used as sensors and probes in life science. Thus, their biophysical properties are well established in solution, while their photophysical properties in cellulo, i. e., in the environment, in which the dyes are designed to function, are generally understood less. To address this issue, we present a sub-ns time-resolved transient absorption study of the excited-state dynamics of a BODIPY-perylene dyad designed as a twisted intramolecular charge transfer (TICT) probe of the local viscosity in live cells.
基于 BODIPY 的给体-受体二聚体广泛用作生命科学中的传感器和探针。因此，它们在溶液中的生物物理性质已经得到很好的确立，而它们在细胞内的光物理性质，即在设计染料发挥作用的环境中，通常理解得较少。为了解决这个问题，我们对设计为活细胞中局部粘度的扭曲分子内电荷转移（TICT）探针的 BODIPY-苝二聚体的激发态动力学进行了亚纳秒时间分辨瞬态吸收研究。
