Engineering Applications of Lasers Department, National Institute of Laser Enhanced Sciences, Cairo University, Giza, 12613, Egypt.
Biophysics Department, Faculty of Science, Cairo University, Giza, 12613, Egypt.
J Fluoresc. 2023 Jul;33(4):1631-1639. doi: 10.1007/s10895-023-03165-8. Epub 2023 Feb 18.
Olive oils are more expensive compared with other vegetable oils. Therefore, adulterating such expensive oil is prevalent. The traditional methods for olive oil adulteration detection are complex and require pre-analysis sample preparation. Therefore, simple and precise alternative techniques are required. In the present study, the Laser-induced fluorescence (LIF) technique was implemented for detecting alteration and adulteration of olive oil mixed with sunflower or corn oil based on the post-heating emission characteristics. Diode-pumped solid-state laser (DPSS, λ = 405 nm) was employed for excitation and the fluorescence emission was detected via an optical fiber connected to a compact spectrometer. The obtained results revealed alterations in the recorded chlorophyll peak intensity due to olive oil heating and adulteration. The correlation of the experimental measurements was evaluated via partial least-squares regression (PLSR) with an R-squared value of 0.95. Moreover, the system performance was evaluated using receiver operating characteristics (ROC) with a maximum sensitivity of 93%.
橄榄油比其他植物油更昂贵。因此，这种昂贵的油很容易被掺假。传统的橄榄油掺假检测方法复杂，需要进行样品前处理。因此，需要更简单、更精确的替代技术。在本研究中，基于加热后的发射特性，采用激光诱导荧光（LIF）技术来检测橄榄油与葵花籽油或玉米油混合后的变化和掺假情况。采用二极管泵浦固态激光器（DPSS，λ=405nm）进行激发，通过与紧凑型光谱仪相连的光纤检测荧光发射。实验结果表明，由于橄榄油加热和掺假，记录的叶绿素峰强度发生了变化。通过偏最小二乘回归（PLSR）对实验测量结果进行相关性评估，得到的 R-squared 值为 0.95。此外，还使用接收器操作特性（ROC）曲线来评估系统性能，得到的最大灵敏度为 93%。
