使用与红细胞偶联的人单克隆类风湿因子，通过反向被动血凝反应检测热聚集IgG（作为免疫复合物的模型）。
The detection of heat-aggregated IgG (as a model for immune complexes) by reverse passive haemagglutination using a human monoclonal rheumatoid factor coupled to erythrocytes.
作者信息
Welsh C J, Steinitz M, Clark M R, Coombs R R
机构信息
University of Cambridge, Department of Pathology, U.K.
出版信息
J Immunol Methods. 1987 Nov 23;104(1-2):271-4. doi: 10.1016/0022-1759(87)90515-1.
A human monoclonal IgM rheumatoid factor (RF) produced in vitro by an Epstein-Barr virus (EBV)-immortalized cell line was purified by protein A-Sepharose adsorption and coupled by the chromic chloride method to human erythrocytes. The RF-coupled cells were incorporated in reverse passive haemagglutination (RPH) assays to detect immune complexes (IC) using heat-aggregated human IgG as a model system. The sensitivity of the RPH was comparable to an enzyme-linked immunosorbent assay (ELISA) using sheep C1q for the detection of ICs.
一种由爱泼斯坦 - 巴尔病毒（EBV）永生化细胞系体外产生的人单克隆IgM类风湿因子（RF），通过蛋白A - 琼脂糖吸附进行纯化，并采用氯化铬法与人红细胞偶联。将偶联有RF的细胞用于反向被动血凝试验（RPH），以热聚集人IgG作为模型系统检测免疫复合物（IC）。RPH的灵敏度与使用羊C1q检测IC的酶联免疫吸附测定（ELISA）相当。
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