一种使用共价包被人IgG的保存红细胞检测类风湿因子的血凝方法。
A haemagglutination method for detection of rheumatoid factor using preserved erythrocytes covalently coated with human IgG.
作者信息
Assimeh S N, Johnson P M
出版信息
J Immunol Methods. 1980;34(3):205-15. doi: 10.1016/0022-1759(80)90047-2.
The preparation and use of a new haemagglutination assay for rheumatoid factor using preserved erythrocytes covalently coated with human IgG is described. Test serum heat inactivation or adsorption with uncoated erythrocytes is not required. This human IgG-coated erythrocyte agglutination test (H/CEAT) appears stable and specific, is suitable for routine determinations of serum IgM-rheumatoid factor, is simple to perform and compares favourably in these properties with either the Rose-Waaler or latex agglutination assays for rheumatoid factor.
本文描述了一种新型类风湿因子血凝试验的制备及应用，该试验使用共价包被人IgG的保存红细胞。无需对检测血清进行热灭活或用未包被的红细胞吸附。这种人IgG包被红细胞凝集试验（H/CEAT）似乎稳定且特异，适用于血清IgM类风湿因子的常规测定，操作简单，在这些特性方面与类风湿因子的罗斯-瓦勒试验或乳胶凝集试验相比具有优势。
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