由游离寡肽（Gly）5引发的单克隆抗体与葡萄球菌的肽聚糖发生特异性反应。

Monoclonal antibodies evoked by the free oligopeptide (Gly)5 reacting specifically with peptidoglycan from staphylococci.

作者信息

Wergeland H I, Asbakk K B, Haaheim L R

机构信息

Department of Microbiology and Immunology, Gade Institute, University of Bergen, Norway.

出版信息

J Immunol Methods. 1987 Nov 23;104(1-2):57-63. doi: 10.1016/0022-1759(87)90487-x.

DOI:10.1016/0022-1759(87)90487-x

PMID:3680962

Abstract

Murine monoclonal antibodies reactive with the Staphylococcus aureus peptidoglycan (PG) epitope (Gly)5 were obtained using the synthetic oligopeptide (Gly)5 in its free form as immunogen. The selected monoclonal antibodies were of the IgM kappa isotype and reacted specifically with PG from S. aureus and Staphylococcus epidermidis, but gave no reaction with PG from Streptococcus pyogenes, Bacillus subtilis and Micrococcus lysodeikticus. Affinity chromatography showed that the antibodies were reactive with the N-terminus of the (Gly)5 peptide. These monoclonal antibodies can be used for the detection of staphylococcal PG in solution.

摘要

以游离形式的合成寡肽（甘氨酸）5作为免疫原，获得了与金黄色葡萄球菌肽聚糖（PG）表位（甘氨酸）5反应的鼠单克隆抗体。所选单克隆抗体为IgM κ同种型，与金黄色葡萄球菌和表皮葡萄球菌的PG特异性反应，但与化脓性链球菌、枯草芽孢杆菌和溶壁微球菌的PG无反应。亲和层析表明，这些抗体与（甘氨酸）5肽的N端反应。这些单克隆抗体可用于检测溶液中的葡萄球菌PG。