Cheng Shanxia, Li Jianfen
Hospital of Wuhan Polytechnic University, Wuhan, 430023, China.
School of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan, 430023, China.
Biochem Biophys Res Commun. 2023 Apr 16;652:55-60. doi: 10.1016/j.bbrc.2023.02.027. Epub 2023 Feb 16.
Preparation of a supermacromolecular photosensitizer that can stay in the tumor site and exhibits high photoconversion efficiency is useful for improving the efficacy of tumor photodynamic therapy (PDT). In this paper, we prepared tetratroxaminobenzene porphyrin (TAPP) loaded biodegradable silk nanospheres (NSs) and characterized their morphology, optical properties and the singlet oxygen-generating capacity. On this basis, the effect of in vitro photodynamic killing efficacy by as-prepared nanometer micelles was evaluated and the tumor retention ability and tumor killing effect of the nanometer micelles were verified by the co-culture of photosensitizer micelle and tumor cells. The results show that tumor cells were killed well under 660 nm laser irradiation even at a lower concentration of as-prepared TAPP NSs. In addition, due to the excellent safety of as-prepared nanomicelle, they exhibit great potential applications in improved tumor PDT.
制备一种能够滞留在肿瘤部位并具有高光转换效率的超高分子量光敏剂，对于提高肿瘤光动力疗法（PDT）的疗效很有用。在本文中，我们制备了负载四氧代氨基苯卟啉（TAPP）的可生物降解丝纳米球（NSs），并对其形态、光学性质和单线态氧生成能力进行了表征。在此基础上，评估了所制备的纳米胶束的体外光动力杀伤效果，并通过光敏剂胶束与肿瘤细胞的共培养验证了纳米胶束的肿瘤滞留能力和肿瘤杀伤效果。结果表明，即使在较低浓度的所制备的TAPP NSs下，在660 nm激光照射下肿瘤细胞也能被很好地杀死。此外，由于所制备的纳米胶束具有优异的安全性，它们在改进的肿瘤PDT中展现出巨大的潜在应用价值。
