Zhai Hongguo, Wang Yue, Geng Lingjun, Guo Qi, Zhang Yuhao, Yang Qingqing, Sun Xia, Guo Yemin, Zhang Yanyan
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo 255000, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo 255000, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu Zibo 255000, China.
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo 255000, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo 255000, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu Zibo 255000, China.
Food Chem. 2023 Jul 15;414:135708. doi: 10.1016/j.foodchem.2023.135708. Epub 2023 Feb 15.
In this paper, an electrochemiluminescence (ECL) biosensor was constructed using BiS@Au nanoflowers as the based nanomaterial and Au@luminol and CdS QDs as independent ECL emission signal respectively. As the substrate of the working electrode, BiS@Au nanoflowers improved the effective area of electrode and accelerated electron transfer rate between gold nanoparticles and aptamer, provided a good interface environment for the loading of luminescent materials. Then, the Au@luminol functionalized DNA2 probe was used as an independent ECL signal source under positive potential and recognized Cd(II), while the CdS QDs functionalized DNA3 probe was used as an independent ECL signal source under negative potential and recognized ampicillin. The simultaneous detection of Cd(II) and ampicillin in different concentrations are realized. This sensor not only has good selectivity and high sensitivity in real sample detection, but also open up a novel way to construct multi-target ECL biosensor for simultaneous detection.
本文构建了一种电化学发光（ECL）生物传感器，以BiS@Au纳米花作为基底纳米材料，分别以Au@鲁米诺和CdS量子点作为独立的ECL发射信号。作为工作电极的基底，BiS@Au纳米花增大了电极的有效面积，加速了金纳米颗粒与适配体之间的电子转移速率，为发光材料的负载提供了良好的界面环境。然后，Au@鲁米诺功能化的DNA2探针在正电位下作为独立的ECL信号源并识别Cd(II)，而CdS量子点功能化的DNA3探针在负电位下作为独立的ECL信号源并识别氨苄青霉素。实现了对不同浓度的Cd(II)和氨苄青霉素的同时检测。该传感器不仅在实际样品检测中具有良好的选择性和高灵敏度，而且为构建用于同时检测的多靶点ECL生物传感器开辟了一条新途径。
