Iguchi H, Natori S, Nawata H, Kato K, Ibayashi H, Chan J S, Seidah N G, Chrétien M
Department of Biochemistry, National Kyushu Cancer Center, Fukuoka, Japan.
J Neurochem. 1987 Dec;49(6):1810-4. doi: 10.1111/j.1471-4159.1987.tb02440.x.
We observed the presence of the novel pituitary protein "7B2" and its release in the bovine adrenal medulla. The 7B2 concentration (mean +/- SEM) in extracts of the bovine adrenal medulla was 952 +/- 155 pg/mg tissue (n = 6). 7B2 was distributed in the chromaffin granule fraction prepared from the bovine adrenal medulla and was released by high K+ and/or nicotine from cultured cells of the bovine adrenal medulla. Co-release of 7B2 with catecholamine induced by nicotine from the cultured bovine chromaffin cells was also observed. In an analysis of the bovine adrenal medulla chromaffin granule fraction on gel permeation chromatography, there was a major peak with an apparent molecular weight of 45,000, whereas a major peak with an apparent molecular weight of 20,000 was found in that on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. On reverse-phase HPLC, a major peak with a retention time of 35 min was observed in the bovine chromaffin granule fraction and in the bovine anterior pituitary extract. These findings indicate that 7B2 is a secretory protein in the bovine adrenal medulla. The possibility that 7B2 might be released with catecholamine, possibly in response to stress, warrants investigation.
我们观察到新型垂体蛋白“7B2”在牛肾上腺髓质中的存在及其释放情况。牛肾上腺髓质提取物中的7B2浓度（平均值±标准误）为952±155 pg/mg组织（n = 6）。7B2分布于从牛肾上腺髓质制备的嗜铬颗粒组分中，并可被高钾和/或尼古丁从牛肾上腺髓质的培养细胞中释放出来。还观察到尼古丁诱导培养的牛嗜铬细胞中7B2与儿茶酚胺的共同释放。在对牛肾上腺髓质嗜铬颗粒组分进行凝胶渗透色谱分析时，出现了一个表观分子量为45,000的主峰，而在十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳分析中则出现了一个表观分子量为20,000的主峰。在反相高效液相色谱分析中，在牛嗜铬颗粒组分和牛垂体前叶提取物中均观察到一个保留时间为35分钟的主峰。这些发现表明7B2是牛肾上腺髓质中的一种分泌蛋白。7B2可能与儿茶酚胺一起释放，可能是对应激的反应，这一可能性值得研究。
