Hummon M R, Costello W J
Department of Zoological and Biomedical Sciences/College of Osteopathic Medicine, Ohio University, Athens 45701.
J Neurosci. 1987 Nov;7(11):3633-8. doi: 10.1523/JNEUROSCI.07-11-03633.1987.
Temperature-sensitive mutants permit the selective expression of mutant genotype. The Drosophila ts mutant shibire (shi) is paralytic at 30 degrees C; the probable primary effect of the mutation is disruption of membrane recycling. In studying the development of the giant fiber (GF) pathway during the pupal period, we find that shi flies exposed to heat pulse during early pupal states exhibit perturbation in the development of an identified neuron that links giant fibers to motoneurons of indirect flight muscles. Concomitantly, latency in activation of these muscles by the giant fiber pathway is significantly increased. Flies exposed to heat pulse during the late pupal period remain similar to control shi and wildtype flies in giant fiber pathway anatomy and muscle latency. Thus, the critical period of development of an identified neuron in a known motor pathway can be defined by its period of sensitivity to the shi defect. The time-dependent defect is apparently specific for cells that are at a developmental stage that is greatly dependent on membrane recycling processes. Use of this mutant will allow us to investigate the possible role of membrane recycling in development and to establish critical periods of neuronal development.
温度敏感突变体允许突变基因型的选择性表达。果蝇的温度敏感突变体“麻痹（shi）”在30摄氏度时会麻痹；该突变的可能主要影响是膜循环的破坏。在研究蛹期巨纤维（GF）通路的发育过程中，我们发现，在蛹早期暴露于热脉冲的shi果蝇，在一个将巨纤维与间接飞行肌运动神经元相连的已鉴定神经元的发育中表现出扰动。与此同时，巨纤维通路激活这些肌肉的潜伏期显著增加。在蛹后期暴露于热脉冲的果蝇，在巨纤维通路解剖结构和肌肉潜伏期方面与对照shi果蝇和野生型果蝇相似。因此，已知运动通路中一个已鉴定神经元的关键发育时期可以由其对shi缺陷的敏感时期来定义。这种时间依赖性缺陷显然对处于极大依赖膜循环过程的发育阶段的细胞具有特异性。使用这种突变体将使我们能够研究膜循环在发育中的可能作用，并确定神经元发育的关键时期。
