Sun Xiaoying, Wan Xinxing, Khan Md Asaduzzaman, Zhang Keke, Yi Xuan, Wang Zhouqi, Chen Ke
Department of Endocrinology, The Third Xiangya Hospital of Central South University, Changsha, People's Republic of China.
The Research Centre for Preclinical Medicine, Southwest Medical University, Luzhou, People's Republic of China.
Diabetes Metab Syndr Obes. 2023 Feb 15;16:469-478. doi: 10.2147/DMSO.S398620. eCollection 2023.
Brown adipose tissue (BAT) can rapidly generate heat and improve energy metabolism. Circular RNAs (circRNAs) are cellular endogenous non-coding RNAs, which can regulate the development and progress of different diseases. However, the role of circRNAs in human BAT is not fully understood. Here, we analyzed the differentially expressed circRNAs (DECs) in human BAT, as well as in white adipose tissue (WAT), and identified new biomarkers of BAT.
Three human BAT and three human subcutaneous WAT samples were selected, and circRNA microarray was performed. Additionally, quantitative real-time polymerase chain reaction (qRT-PCR) was applied to determine the expression of six circRNAs. Finally, the functional analysis was performed by bioinformatics.
Compared to WAT, 152 upregulated circRNAs and 201 downregulated circRNAs were identified in BAT. The DECs were further subjected to GO and KEGG enrichment analysis. Several circRNAs, for example, hsa_circ_0006168, hsa_circ_26337 and hsa_circ_0007507 were found upregulated and hsa_circ_0030162 was found downregulated in human BAT compared to WAT.
This study profiles the circRNA expression in human BAT and WAT, and suggests hsa_circ_0006168, hsa_circ_26337, hsa_circ_0007507, and hsa_circ_0030162 as novel biomarkers for human BAT.
棕色脂肪组织（BAT）可快速产热并改善能量代谢。环状RNA（circRNA）是细胞内源性非编码RNA，可调节不同疾病的发生发展。然而，circRNA在人类BAT中的作用尚未完全明确。在此，我们分析了人类BAT以及白色脂肪组织（WAT）中差异表达的circRNA（DECs），并鉴定出BAT的新生物标志物。
选取3例人类BAT样本和3例人类皮下WAT样本，进行circRNA微阵列分析。此外，应用定量实时聚合酶链反应（qRT-PCR）检测6种circRNA的表达。最后，通过生物信息学进行功能分析。
与WAT相比，在BAT中鉴定出15上调的circRNA和201种下调的circRNA。对这些DECs进一步进行基因本体（GO）和京都基因与基因组百科全书（KEGG）富集分析。与WAT相比，发现几种circRNA，如hsa_circ_0006168、hsa_circ_26337和hsa_circ_0007507在人类BAT中上调，而hsa_circ_0030162下调。
本研究分析了人类BAT和WAT中的circRNA表达情况，并提示hsa_circ_0006168、hsa_circ_26337、hsa_circ_0007507和hsa_circ_0030162可作为人类BAT的新型生物标志物。
