水培和 LED 光照条件下生长的牛至（ Lippia palmeri S. Watson ）叶精油的抗增殖作用。

Antiproliferative Effect of Essential Oil Obtained from Oregano (Lippia palmeri S. Watson) Leaves Grown in Hydroponics and LED Light.

机构信息

Departamento de Investigaciones Científicas y Tecnológicas (DICTUS), Edificio 7G, Universidad de Sonora, Avenida Luis Donaldo Colosio s/n Edificio 7G, Centro, C.P., 83000, Hermosillo, Sonora, México.

Centro de investigación en Alimentación y Desarrollo A.C. Grupo de investigación en polímeros, Carretera Gustavo Enrique Astiazarán Rosas No. 46, Col. La Victoria, C.P., 83304, Hermosillo, Sonora, México.

出版信息

Chem Biodivers. 2023 Apr;20(4):e202201076. doi: 10.1002/cbdv.202201076. Epub 2023 Mar 10.

DOI:10.1002/cbdv.202201076

PMID:36815541

Abstract

Nowadays, light-emitting diodes (LED) provide an alternative source to sunlight with specific intensity and wavelength that promotes plant growth. The features offered by LED could also stimulate the production of secondary metabolites of pharmaceutical interest. This work analyzed the cultivation of oregano (Lippia palmeri S. Watson) in a floating root hydroponic system supplemented by full-spectrum LED artificial light. Growth indicators like height, diameter, number of shoots, and leaf length and width were measured. The essential oil (EO) composition from the leaves of wild and hydroponic conditions found thymol (41.8 %) as the main product for the former and carvacrol (47 %) in hydroponics. The antiproliferative activity of EOs on human colorectal cancer HCT-15 shows that 6.4 μg/ml for hydroponic and 7.4 μg/ml for the wild plant reduce more than 50 % the cell viability. Overall, this study indicates that hydroponic conditions and full spectrum LED modifies the composition of the EO of L. palmeri on compared with the wild plant, which effectively induces cell growth inhibition in human colorectal cancer.

摘要

如今，发光二极管（LED）提供了一种替代阳光的光源，具有特定的强度和波长，可促进植物生长。LED 提供的功能还可以刺激具有药物应用价值的次生代谢产物的产生。本研究分析了在全光谱 LED 人工光补充的漂浮根水培系统中种植牛至（Lippia palmeri S. Watson）的情况。测量了高度、直径、新芽数量以及叶片长度和宽度等生长指标。从野生和水培条件下的叶片中提取的精油（EO）成分发现，前者的主要产物为百里香酚（41.8％），而水培条件下的主要产物为香芹酚（47％）。EO 对人结肠癌细胞 HCT-15 的抗增殖活性表明，水培条件下的 6.4μg/ml 和野生植物中的 7.4μg/ml 可使细胞活力降低超过 50％。总体而言，这项研究表明，与野生植物相比，水培条件和全光谱 LED 会改变 L. palmeri 的 EO 组成，而这有效地诱导了人结肠癌细胞的生长抑制。