Krakowska Anna, Cedzyński Maciej, Wosiak Agnieszka, Swiechowski Rafal, Krygier Adrian, Tkaczyk Marcin, Zeman Krzysztof
Department of Paediatrics, Immunology and Nephrology, Polish Mother's Memorial Hospital Research Institute, Lodz, Poland.
Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland.
Cent Eur J Immunol. 2022;47(3):260-266. doi: 10.5114/ceji.2022.119625. Epub 2022 Sep 21.
Toll-like receptors (TLRs) contribute to the innate immune system. They are an element of non-specific immunity, which enables organisms to react quickly to foreign antigens, without being previously exposed to them. TLRs are pattern recognition receptors. TLR gene polymorphisms are widely investigated in connection with various infections. The aims of the study were: to investigate the role of TLR2 and TLR4 polymorphisms in the course of urinary tract infections (UTIs); to test for differences in distribution of these polymorphisms between children with urinary tract malformations suffering from recurrent UTI (rUTI), children with malformations but without rUTI and healthy controls; to determine whether these polymorphisms predispose to rUTI; and to analyse how polymorphisms and urine neutrophil gelatinase-associated lipocalin (NGAL) and interleukin 8 (IL-8) concentrations affect one another.
The group consisted of 133 children (1-18 years old), 68 female and 65 male. The group was divided into 4 subgroups: A (rUTI with urinary tract malformations), B (urinary tract malformations without rUTI), C (rUTI) and D (healthy controls). Polymorphisms were analysed using PCR-RFLP. IL-8 and NGAL urine concentrations were established using immunoenzymatic methods.
TLR2 Arg753Gln and TLR4 Arg299Gly appeared significantly more often among children with rUTI. No correlation between urine IL-8 and urine NGAL and polymorphisms was found. Urine NGAL concentration was significantly higher among children with urinary tract malformations.
TLR2 Arg753Gln and TLR4 Asp299Gly may predispose to rUTI. Urine NGAL concentration suggests the presence of kidney tissue injury, of varying degrees, among children with urinary tract malformations.
Toll样受体（TLR）有助于先天免疫系统。它们是非特异性免疫的一个组成部分，使生物体能够对外来抗原迅速做出反应，而无需事先接触过这些抗原。TLR是模式识别受体。TLR基因多态性与各种感染相关的研究广泛开展。本研究的目的是：探讨TLR2和TLR4多态性在尿路感染（UTI）病程中的作用；检测复发性UTI（rUTI）的尿路畸形患儿、有畸形但无rUTI的患儿及健康对照之间这些多态性分布的差异；确定这些多态性是否易导致rUTI；分析多态性与尿中性粒细胞明胶酶相关脂质运载蛋白（NGAL）和白细胞介素8（IL-8）浓度之间如何相互影响。
该组由133名儿童（1至18岁）组成，其中68名女性，65名男性。该组分为4个亚组：A组（有尿路畸形的rUTI）、B组（有尿路畸形但无rUTI）、C组（rUTI）和D组（健康对照）。使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析多态性。采用免疫酶法测定尿IL-8和NGAL浓度。
TLR2 Arg753Gln和TLR4 Arg299Gly在rUTI患儿中出现的频率明显更高。未发现尿IL-8和尿NGAL与多态性之间存在相关性。尿路畸形患儿的尿NGAL浓度明显更高。
TLR2 Arg753Gln和TLR4 Asp299Gly可能易导致rUTI。尿NGAL浓度提示尿路畸形患儿存在不同程度的肾组织损伤。
