Yao Xinyu, Wang Wenzhu, Li Yue, Cao Zhendong, Wang Yongheng, Yuan Yi, Li Xiaoling, Liang Xin, Liu Lanxiang, Yu Yan
Graduate School of Chengde Medical University, Hebei Province, China.
First Hospital of Qinhuangdao, Hebei Province, China.
Data Brief. 2023 Feb 1;47:108947. doi: 10.1016/j.dib.2023.108947. eCollection 2023 Apr.
Here, we treated moderately traumatic brain injury (TBI) rats with different modalities, including transplantation with mesenchymal stem cells (MSCs), treatment with low-intensity transcranial ultrasound stimulation (LITUS), and a combination of the two. After the TBI rat model was established, MSCs ( injection within 24 h after injury), LITUS (continuous uninterrupted treatment for 28 days) or combined MSCs + LITUS were administered, and mNSS score, performance of behavior and multiple protein levels were compared between groups by behavioral observation, neurological function assessment and pathological analysis. Nestin, neuron-specific enolase (NSE), growth-associated protein 43 (GAP-43) and postsynaptic density protein (PSD-95) were significantly increased and glial fibrillary acidic protein (GFAP) was significantly decreased in the hippocampus of rats in the combination treatment group; brain-derived neurotrophic factor (BDNF), tumor necrosis factor-α (TNF-α) and aquaporin-4 (AQP-4) were significantly decreased in the injured peripheral cortex. The result of mNSS scores was: TBI group > LITUS group > MSCs group > MSCs+LITUS group > sham group. The alternate correct rate of Y-maze was: sham group > MSCs+LITUS group > MSCs group > LITUS group > TBI group. This data compares the efficacy of MSCs, LITUS, and combination therapy on the level expression of stem cell differentiation related proteins, synaptic plasticity-related proteins, neurotrophic factors, inflammatory factors, and edema-related proteins after TBI by quantitative pathological examination. For a complete description, interpretation, and discussion of the data refer to the article in press [1].
在此，我们采用不同方式治疗中度创伤性脑损伤（TBI）大鼠，包括间充质干细胞（MSCs）移植、低强度经颅超声刺激（LITUS）治疗以及两者联合治疗。建立TBI大鼠模型后，给予MSCs（损伤后24小时内注射）、LITUS（连续不间断治疗28天）或联合MSCs + LITUS治疗，通过行为观察、神经功能评估和病理分析比较各组的mNSS评分、行为表现和多种蛋白质水平。联合治疗组大鼠海马中巢蛋白、神经元特异性烯醇化酶（NSE）、生长相关蛋白43（GAP - 43）和突触后致密蛋白（PSD - 95）显著增加，胶质纤维酸性蛋白（GFAP）显著降低；损伤外周皮质中脑源性神经营养因子（BDNF）、肿瘤坏死因子-α（TNF - α）和水通道蛋白4（AQP - 4）显著降低。mNSS评分结果为：TBI组＞LITUS组＞MSCs组＞MSCs + LITUS组＞假手术组。Y迷宫交替正确率为：假手术组＞MSCs + LITUS组＞MSCs组＞LITUS组＞TBI组。本数据通过定量病理检查比较了MSCs、LITUS及联合治疗对TBI后干细胞分化相关蛋白、突触可塑性相关蛋白、神经营养因子、炎症因子和水肿相关蛋白水平表达的疗效。有关数据的完整描述、解释和讨论请参考即将发表的文章[1]。
