赤蚯蚓血红蛋白晶体。

Crystals of Lumbricus erythrocruorin.

作者信息

Royer W E, Hendrickson W A, Love W E

机构信息

Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032.

出版信息

J Mol Biol. 1987 Sep 5;197(1):149-53. doi: 10.1016/0022-2836(87)90618-8.

DOI:10.1016/0022-2836(87)90618-8

PMID:3681992

Abstract

Lumbricus terrestris erythrocruorin, a 3.9 X 10(6) Mr respiratory protein, has been crystallized in four different forms. Despite the high molecular symmetry apparent from images in electron micrographs, only one crystal form expresses any molecular symmetry as crystallographic symmetry. The lattice parameters provide upper limits on the molecular dimensions of 267 A X 308 A X 172 A (1 A = 0.1 nm), which agree well with dimensions obtained from electron micrographs of negatively stained molecules. We have collected diffraction data to 5.5 A from type III crystals and have begun a structural analysis.

摘要

蚯蚓血红蛋白，一种分子量为3.9×10⁶的呼吸蛋白，已结晶为四种不同形式。尽管从电子显微镜图像中可明显看出其具有高度的分子对称性，但只有一种晶体形式表现出任何作为晶体学对称性的分子对称性。晶格参数给出了分子尺寸的上限，为267 Å×308 Å×172 Å（1 Å = 0.1 nm），这与从负染分子的电子显微镜图像获得的尺寸非常吻合。我们已从III型晶体收集了分辨率达5.5 Å的衍射数据，并已开始进行结构分析。