Development of an analytical chip for colorimetric detection of medium-chain aldehydes by reaction with pararosaniline in porous glass.

Medium-chain aldehydes are common human biogases that can be detected in the breath of patients with lung diseases. As such, the measurement of medium-chain aldehyde gases in human breath can provide significant, noninvasive, and diagnostic information related to the potential presence of such diseases. In this study, an analytical chip is developed for the detection of medium-chain aldehydes without interference from short-chain aldehydes. This analytical chip is composed of porous glass impregnated with pararosaniline and an acid (i.e., acetic acid with small amount of phosphoric acid). After exposure to medium-chain aldehydes, the red analytical chip became violet in color, and an absorption peak was observed at 620 nm. It was found that a non-reversible reaction occurred in the porous glass, therefore, the analytical chip functions in a cumulative manner. A linear relationship was determined between the absorbance change of the analytical chip at 620 nm and the nonanal exposure concentration. Importantly, the developed analytical chip successfully detected nonanal at concentrations of 8-270 ppb as calculated from the absorbance change at 620 nm after a 24 h exposure time. In addition, nonanal concentration was estimated using the change in the R value of the analytical chip photograph. This method is suitable for point-of-care breath analysis. Finally, the analytical chip was also found to be active toward octanal and decanal with a relative sensitivity of 0.7 compared to that of nonanal; it was not active toward short-chain aldehydes.