Batalha Isadora Maria, Maylem Excel Rio S, Spicer Leon J, Pena Bello Camilo Andres, Archilia Evandro Carlos, Schütz Luis Fernando
Departament of Agriculture, Veterinary, and Rangeland Sciences, University of Nevada, Reno, NV 89557, USA.
Department of Animal and Food Sciences, Oklahoma State University Stillwater, OK 74078, USA.
Mol Cell Endocrinol. 2023 Apr 5;565:111890. doi: 10.1016/j.mce.2023.111890. Epub 2023 Feb 21.
Asprosin is an adipokine synthesized by the white adipose tissue that regulates glucose homeostasis and that has been reported to affect bovine theca cell function and follicular growth, but its role on granulosa cell functions remains to be unveiled. Hence, the objective of this study was to investigate asprosin impacts on granulosa cell steroidogenesis. Bovine granulosa cells from small ovarian follicles were cultured in vitro to investigate the effects of asprosin on cell proliferation, production of steroids, mRNA abundance of genes that encode steroidogenic enzymes and cell cycle regulators, and protein relative abundance of steroidogenic signaling pathways. Asprosin was shown to affect granulosa cell functions in a dose-dependent manner. In the presence of FSH, asprosin enhanced estradiol production and stimulated an increase in mRNA expression of FSHR and CYP19A1 in a dose-dependent manner. In the presence of IGF1, asprosin decreased estradiol production, increased progesterone production, altered PKA relative protein expression, and tended to alter the ratio of p-ERK1/2/total ERK1/2 protein expression in a dose-dependent manner. Furthermore, asprosin increased p-53 gene expression in basal culture conditions and with or without FSH and IGF1. Taken together, findings of this study show that asprosin is a regulator of granulosa cell functions and the effects of asprosin depend on dose and cell culture conditions.
脂联素是一种由白色脂肪组织合成的脂肪因子，它调节葡萄糖稳态，据报道会影响牛卵泡膜细胞功能和卵泡生长，但其对颗粒细胞功能的作用仍有待揭示。因此，本研究的目的是探讨脂联素对颗粒细胞类固醇生成的影响。从小卵泡中分离出牛颗粒细胞进行体外培养，以研究脂联素对细胞增殖、类固醇生成、编码类固醇生成酶和细胞周期调节因子的基因的mRNA丰度以及类固醇生成信号通路的蛋白质相对丰度的影响。结果表明，脂联素以剂量依赖的方式影响颗粒细胞功能。在促卵泡素（FSH）存在的情况下，脂联素以剂量依赖的方式增强雌二醇的生成，并刺激FSH受体（FSHR）和细胞色素P450芳香化酶19A1（CYP19A1）的mRNA表达增加。在胰岛素样生长因子1（IGF1）存在的情况下，脂联素降低雌二醇的生成，增加孕酮的生成，改变蛋白激酶A（PKA）的相对蛋白表达，并倾向于以剂量依赖的方式改变磷酸化细胞外信号调节激酶1/2（p-ERK1/2）与总细胞外信号调节激酶1/2（total ERK1/2）蛋白表达的比值。此外，在基础培养条件下以及有或没有FSH和IGF1的情况下，脂联素都会增加p-53基因的表达。综上所述，本研究结果表明脂联素是颗粒细胞功能的调节因子，其作用取决于剂量和细胞培养条件。
