Department of Biology, Graduate School of Science and Engineering, Chiba University, 1-33 Yayoi-chou, Inage-ku, Chiba 263-8522, Japan.
Department of Biology, Graduate School of Science, Chiba University, 1-33 Yayoi-chou, Inage-ku, Chiba 263-8522, Japan.
STAR Protoc. 2023 Mar 17;4(1):102085. doi: 10.1016/j.xpro.2023.102085. Epub 2023 Feb 2.
The budding yeast Saccharomyces cerevisiae is an excellent model for examining the effects of ploidy. Here, we provide a protocol for producing polyploid cells by creating a basic unit (matΔ) and polyploidizing it via repeated mating. We describe steps for basic unit construction by one-step transformation, increased ploidy via repeated mating, and ploidy confirmation using flow cytometry. This protocol can be broadly applied to evaluate the physiology of polyploid cells. For complete details on the use and execution of this protocol, please refer to Oya and Matsuura (2022)..
budding 酵母酿酒酵母是研究倍性影响的极好模型。在这里，我们提供了一种通过创建基本单元 (matΔ) 并通过多次交配使其多倍化来产生多倍体细胞的方案。我们描述了通过一步转化构建基本单元、通过多次交配增加倍性以及使用流式细胞术确认倍性的步骤。该方案可广泛用于评估多倍体细胞的生理学。有关此方案的使用和执行的完整详细信息，请参阅 Oya 和 Matsuura (2022)。
