Farc Ovidiu, Budisan Liviuta, Berindan-Neagoe Ioana, Braicu Cornelia, Zanoaga Oana, Zaharie Florin, Cristea Victor
Immunology Department, "Iuliu Hatieganu" University of Medicine and Pharmacy, 400347 Cluj-Napoca, Romania.
Research Center for Functional Genomics, Biomedicine and Translational Medicine "Iuliu Hatieganu" University of Medicine and Pharmacy, 400347 Cluj-Napoca, Romania.
Curr Issues Mol Biol. 2023 Jan 20;45(2):975-989. doi: 10.3390/cimb45020063.
MicroRNAs (miRNAs) are molecules with a role in the post-transcriptional regulation of messenger RNA, being involved in a wide range of biological and pathological processes. In the present study, we aim to characterize the behavior of a few miRNAs with roles in the cell cycle and differentiation of colon cancer (CC) cells. The present work considers miRNAs as reflections of the complex cellular processes in which they are generated, their observed variations being used to characterize the molecular networks in which they are part and through which cell proliferation is achieved. Tumoral and adjacent normal tissue samples were obtained from 40 CC patients, and the expression of miR-29a, miR-146a, miR-215 and miR-449 were determined by qRT-PCR analysis. Subsequent bioinformatic analysis was performed to highlight the transcription factors (TFs) network that regulate the miRNAs and functionally characterizes this network. There was a significant decrease in the expression of all miRNAs in tumor tissue. All miRNAs were positively correlated with each other. The analysis of the TF network showed tightly connected functional modules related to the cell cycle and associated processes. The four miRNAs are downregulated in CC; they are strongly correlated, showing coherence within the cellular network that regulates them and highlighting possible approach strategies.
微小RNA（miRNA）是一类在信使核糖核酸转录后调控中发挥作用的分子，参与广泛的生物学和病理过程。在本研究中，我们旨在表征几种在结肠癌（CC）细胞周期和分化中起作用的miRNA的行为。本研究将miRNA视为其产生过程中复杂细胞过程的反映，通过观察它们的变化来表征它们所属的分子网络以及实现细胞增殖的途径。从40例CC患者中获取肿瘤及相邻正常组织样本，通过qRT-PCR分析确定miR-29a、miR-146a、miR-215和miR-449的表达。随后进行生物信息学分析，以突出调控这些miRNA的转录因子（TF）网络，并对该网络进行功能表征。肿瘤组织中所有miRNA的表达均显著降低。所有miRNA之间呈正相关。TF网络分析显示与细胞周期及相关过程相关的紧密连接的功能模块。这四种miRNA在CC中表达下调；它们高度相关，在调控它们的细胞网络中表现出一致性，并突出了可能的研究策略。
