Cortijo J, Dixon J S, Foster R W, Small R C
Department of Physiological Sciences, Medical School, University of Manchester, United Kingdom.
J Pharmacol Methods. 1987 Nov;18(3):253-66. doi: 10.1016/0160-5402(87)90075-1.
Electron microscopy showed that the process of skinning guinea pig trachaelis muscle using Triton X-100 destroys the plasma membranes and causes some retraction of the myofibrils toward the center of the cells. Seven days represents an optimal period for the low temperature storage of the skinned fibers. Increasing the imposed tension from 0.25 to 1.0 g reduces the size of the maximal response to Ca2+. Some sensitization of the skinned fibers to Ca2+ follows the construction of the initial Ca2+ log concentration-response curve. Exogenous calmodulin is not essential for Ca2+-induced tension development and, at 20 degrees C, does not potentiate Ca2+. Useful economy can be achieved without compromising Ca2+ sensitivity or responsiveness by using ATP (a component of the relaxing solution) of purity 99% rather than of 99-100%.
电子显微镜显示，用曲拉通X-100剥除豚鼠气管肌的过程会破坏质膜，并导致肌原纤维向细胞中心发生一定程度的收缩。七天是剥除纤维低温保存的最佳时间。将施加的张力从0.25克增加到1.0克会降低对Ca2+的最大反应幅度。在构建初始Ca2+对数浓度-反应曲线后，剥除的纤维对Ca2+会有一定程度的敏感化。外源性钙调蛋白对于Ca2+诱导的张力发展并非必需，并且在20摄氏度时不会增强Ca2+的作用。使用纯度为99%而非99 - 100%的ATP（松弛溶液的一种成分），在不影响Ca2+敏感性或反应性的情况下可以实现有效节约。
