Nguyen Marina Minh, Karboune Salwa
Department of Food Science and Agricultural Chemistry, Macdonald Campus, McGill University, Ste Anne de Bellevue, QC H9X3V9, Canada.
Antioxidants (Basel). 2023 Feb 15;12(2):486. doi: 10.3390/antiox12020486.
With the aim to develop essential oil (EO) multi-antioxidant systems, combinatorial interactions of selected phenol and terpene-rich EOs (from Pimento Berry, Ceylon Cinnamon, Clove, Sage, White thyme; Oregano) enriched with individual polyphenols, crude plant extracts, and mixtures of their major polyphenols were investigated using single electron transfer (SET)-based DPPH and hydrogen atom transfer (HAT)-based ORAC assays. Polyphenols that enriched Eos the most favorably were rosmarinic acid (IC of 0.0891-0.1448 mg enriched EO/mg DPPH; 5772-17,879 µmol TE/g enriched EO) and quercetin (IC of 0.0682-0.1060 mg enriched EO/mg DPPH; Trolox Equivalents (TE) of 9776-14,567µmol /g enriched EO), whereas -coumaric acid (IC of 0.1865-1.1424 mg enriched EO/mg DPPH; 7451.00-11,588 µmol TE/g enriched EO) and rutin hydrate (IC of 0.1140-0.3112 mg enriched EO/mg DPPH; 2298-6227 µmol TE/g enriched EO) were the least favorable. Enrichments with polyphenol mixes and crude extracts exhibited synergistic and additive effects in the SET-based DPPH assay. In the HAT-based ORAC assay, EO enrichments with crude extracts exhibited more additive effects, as well as less antagonistic effects, than enrichments with their major polyphenol mixes, revealing the significant contributions of minor compounds. EOs enriched with crude green tea and apple extracts exhibited synergistic or additive effects, whereas EOs enriched with grape seed and rosemary extracts exhibited equal antagonistic effects. Predictive models were developed to explain the variability between the observed and predicted antioxidant activities of enriched EOs.
为了开发精油（EO）多抗氧化系统，使用基于单电子转移（SET）的DPPH和基于氢原子转移（HAT）的ORAC测定法，研究了富含单个多酚、粗植物提取物及其主要多酚混合物的选定富含酚类和萜类的精油（来自多香果浆果、锡兰肉桂、丁香、鼠尾草、白百里香；牛至）的组合相互作用。对精油最有利的多酚是迷迭香酸（富集精油的IC为0.0891 - 0.1448 mg富集精油/mg DPPH；5772 - 17,879 μmol TE/g富集精油）和槲皮素（富集精油的IC为0.0682 - 0.1060 mg富集精油/mg DPPH；9776 - 14,567 μmol TE/g富集精油），而反式香豆酸（富集精油的IC为0.1865 - 1.1424 mg富集精油/mg DPPH；7451.00 - 11,588 μmol TE/g富集精油）和芦丁水合物（富集精油的IC为0.1140 - 0.3112 mg富集精油/mg DPPH；2298 - 6227 μmol TE/g富集精油）最不利。在基于SET的DPPH测定中，用多酚混合物和粗提取物进行富集表现出协同和加和效应。在基于HAT的ORAC测定中，与用主要多酚混合物进行富集相比，用粗提取物对精油进行富集表现出更多的加和效应以及更少的拮抗效应，揭示了次要化合物的重要贡献。富含粗绿茶和苹果提取物的精油表现出协同或加和效应，而富含葡萄籽和迷迭香提取物的精油表现出同等的拮抗效应。建立了预测模型来解释富集精油的观察到的和预测的抗氧化活性之间的变异性。
