Benedek Ildikó, Altbäcker Vilmos, Zsolnai Attila, Nagy István, Mezőszentgyörgyi Dávid, Molnár Tamás
Department of Animal Breeding, Institute of Animal Breeding Sciences, Hungarian University of Agriculture and Life Sciences, 7400 Kaposvar, Hungary.
Department of Nature Conservation, Institute for Wildlife Management and Nature, Hungarian University of Agriculture and Life Sciences, 7400 Kaposvar, Hungary.
Animals (Basel). 2023 Feb 15;13(4):671. doi: 10.3390/ani13040671.
One of the problematic points of rabbit breeding is that the nutritional requirements of the kits are not fully satisfied by the does' milk production from the third week of lactation onwards. The prolactin receptor gene has a significant effect on reproductive processes, and its polymorphisms have been associated with milk production in several species (cattle, goats, sheep, and buffalo). The European wild rabbit (), has a more diverse genetic background compared to domesticated lines. In the course of our study, sequencing of the 1210 bp long segment of the PRLR gene promoter region was accomplished. We detected four point mutations (SNP1-407G > A, SNP2-496G > C, SNP3-926T> and SNP4-973A > C) and one microsatellite at position 574. In our population, the four SNPs were segregated into four genotypes: AACCCCCC, GGGGTTAA, AAGGTTAC, and GGGGTCAC. Our results show that the genotype in the homozygous form is associated with higher milk production (1564.7 ± 444.7 g) compared to the other three genotypes (AACCCCCC 1399.1 ± 326.8 g; GTGACCTT 1403.8 ± 517.1 g; GGGGTCAC 1220.0 ± 666.2 g), and the short microsatellite repeat (167 bp) also coincides with significantly higher milk production (1623.8 ± 525.1 g). These results make the marker-assisted selection possible also for domesticated lines.
家兔养殖的问题之一是，从哺乳期第三周起，母兔的产奶量无法完全满足幼兔的营养需求。催乳素受体基因对繁殖过程有显著影响，其多态性与多个物种（牛、山羊、绵羊和水牛）的产奶量相关。与家养品系相比，欧洲野兔具有更多样化的遗传背景。在我们的研究过程中，完成了催乳素受体基因（PRLR）启动子区域1210 bp长片段的测序。我们检测到四个点突变（SNP1 - 407G > A、SNP2 - 496G > C、SNP3 - 926T>和SNP4 - 973A > C）以及位于574位置的一个微卫星。在我们的群体中，这四个单核苷酸多态性（SNP）分为四种基因型：AACCCCCC、GGGGTTAA、AAGGTTAC和GGGGTCAC。我们的结果表明，与其他三种基因型（AACCCCCC为1399.1 ± 326.8 g；GTGACCTT为1403.8 ± 517.1 g；GGGGTCAC为1220.0 ± 666.2 g）相比，纯合形式的基因型与更高的产奶量（1564.7 ± 444.7 g）相关，并且短微卫星重复序列（167 bp）也与显著更高的产奶量（1623.8 ± 525.1 g）相符。这些结果使得家养品系也能够进行标记辅助选择。
