Geronikolou Styliani A, Vasdekis Vasilis, Mantzou Aimilia, Davos Constantinos, Cokkinos Dennis V, Chrousos George P
Clinical, Translational Research and Experimental Surgery Centre, Biomedical Research Foundation of the Academy of Athens, 4, Soranou Ephessiou Str., 11527 Athens, Greece.
First Department of Pediatrics, National and Kapodistrian University of Athens Medical School, "Aghia Sophia" Children's Hospital, Thivon 1, 11527 Athens, Greece.
Children (Basel). 2023 Jan 30;10(2):248. doi: 10.3390/children10020248.
This pilot repeated measures study aims to evaluate the dynamics of the autonomic nervous system (ANS), the hypothalamic-pituitary-adrenal (HPA) axis, and/or their interplay with low-level inflammation in healthy schoolchildren during consecutive extrinsic stimuli. Twenty healthy schoolchildren and adolescents aged 11-14 years (12.5 ± 1.5) were consecutively exposed to an oral task (#2) and an arithmetic task (#3) (Trier Social Stress Test for Children (TSST-C)), lasting 5 min each, and a three-minute cellular phone call (#4). Salivary cortisol (SC) was sampled at baseline (#1) and immediately after each exposure (#2, 3, and 4). Baseline serum high-sensitivity C-reactive protein (hsCRP) and cortisol levels were also assessed. ANS dynamics and complexity were measured using Sample Entropy (SampEn) at each experimental time period (#1-4). Baseline serum hCRP and cortisol correlated negatively to each other, while the ANS and HPA axis acute reactions to the three consecutive stimuli differed over time. The ANS adaptation to these stimuli included complexity modulation, which was not dependent on baseline hsCRP or cortisol, and weakened during the third stimulation. However, baseline hsCRP and cortisol had a weakening and an increasing effect on the HPA axis over time, respectively. We conclude that low-level inflammation and baseline morning cortisol level have no effect on ANS dynamics but influence the HPA axis response to consecutive external stimuli.
这项试点重复测量研究旨在评估健康学童在连续外部刺激期间自主神经系统（ANS）、下丘脑-垂体-肾上腺（HPA）轴的动态变化，以及/或者它们与低水平炎症之间的相互作用。20名年龄在11 - 14岁（12.5±1.5）的健康学童和青少年连续接受一项口头任务（#2）和一项算术任务（#3）（儿童特里尔社会应激测试（TSST-C）），每项任务持续5分钟，以及一个三分钟的手机通话（#4）。在基线时（#1）以及每次暴露后立即（#2、3和4）采集唾液皮质醇（SC）样本。还评估了基线血清高敏C反应蛋白（hsCRP）和皮质醇水平。在每个实验时间段（#1 - 4）使用样本熵（SampEn）测量ANS的动态变化和复杂性。基线血清hCRP和皮质醇彼此呈负相关，而ANS和HPA轴对这三个连续刺激的急性反应随时间不同。ANS对这些刺激的适应性包括复杂性调节，其不依赖于基线hsCRP或皮质醇，并且在第三次刺激期间减弱。然而，基线hsCRP和皮质醇分别随着时间对HPA轴有减弱和增强作用。我们得出结论，低水平炎症和基线早晨皮质醇水平对ANS动态变化没有影响，但会影响HPA轴对连续外部刺激的反应。
