• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

牛卵丘细胞中与 DNA 双链断裂相关的非编码 RNA 竞争性内源性 RNA 网络。

DNA Double-Strand Break-Related Competitive Endogenous RNA Network of Noncoding RNA in Bovine Cumulus Cells.

机构信息

Department of Laboratory Animals, College of Animal Sciences, Jilin University, Changchun 130062, China.

Experimental Testing Center, Jilin Agricultural Science and Technology University, Jilin 132101, China.

出版信息

Genes (Basel). 2023 Jan 22;14(2):290. doi: 10.3390/genes14020290.

DOI:10.3390/genes14020290
PMID:36833217
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9956238/
Abstract

(1) Background: DNA double strand breaks (DSBs) are the most serious form of DNA damage that affects oocyte maturation and the physiological state of follicles and ovaries. Non-coding RNAs (ncRNAs) play a crucial role in DNA damage and repair. This study aims to analyze and establish the network of ncRNAs when DSB occurs and provide new ideas for next research on the mechanism of cumulus DSB. (2) Methods: Bovine cumulus cells (CCs) were treated with bleomycin (BLM) to construct a DSB model. We detected the changes of the cell cycle, cell viability, and apoptosis to determine the effect of DSBs on cell biology, and further evaluated the relationship between the transcriptome and competitive endogenous RNA (ceRNA) network and DSBs. (3) Results: BLM increased γH2AX positivity in CCs, disrupted the G1/S phase, and decreased cell viability. Totals of 848 mRNAs, 75 long noncoding RNAs (lncRNAs), 68 circular RNAs (circRNAs), and 71 microRNAs (miRNAs) in 78 groups of lncRNA-miRNA-mRNA regulatory networks, 275 groups of circRNA-miRNA-mRNA regulatory networks, and five groups of lncRNA/circRNA-miRNA-mRNA co-expression regulatory networks were related to DSBs. Most differentially expressed ncRNAs were annotated to cell cycle, p53, PI3K-AKT, and WNT signaling pathways. (4) Conclusions: The ceRNA network helps to understand the effects of DNA DSBs activation and remission on the biological function of CCs.

摘要

（1）背景：DNA 双链断裂（DSBs）是影响卵母细胞成熟和卵泡及卵巢生理状态的最严重的 DNA 损伤形式。非编码 RNA（ncRNA）在 DNA 损伤和修复中发挥着至关重要的作用。本研究旨在分析并建立 DSB 发生时 ncRNA 的网络，为下一步研究卵丘 DSB 的机制提供新的思路。

（2）方法：用博来霉素（BLM）处理牛卵丘细胞（CCs）构建 DSB 模型。我们检测细胞周期、细胞活力和细胞凋亡的变化，以确定 DSB 对细胞生物学的影响，进一步评估转录组与竞争内源性 RNA（ceRNA）网络和 DSBs 之间的关系。

（3）结果：BLM 增加了 CCs 中的 γH2AX 阳性，破坏了 G1/S 期，降低了细胞活力。在 78 组 lncRNA-miRNA-mRNA 调控网络、275 组 circRNA-miRNA-mRNA 调控网络和 5 组 lncRNA/circRNA-miRNA-mRNA 共表达调控网络中，共鉴定出 848 个 mRNAs、75 个长非编码 RNA（lncRNA）、68 个环状 RNA（circRNA）和 71 个 microRNA（miRNA）与 DSBs 相关。大多数差异表达的 ncRNA 被注释到细胞周期、p53、PI3K-AKT 和 WNT 信号通路。

（4）结论：ceRNA 网络有助于理解 DNA DSBs 激活和缓解对 CCs 生物学功能的影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d1d/9956238/f22efdccde9d/genes-14-00290-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d1d/9956238/b1e749290bde/genes-14-00290-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d1d/9956238/149e5ed133c5/genes-14-00290-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d1d/9956238/b1560f669019/genes-14-00290-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d1d/9956238/1d7bce6d2b78/genes-14-00290-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d1d/9956238/ce392b11e3fc/genes-14-00290-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d1d/9956238/fc8c699bccbe/genes-14-00290-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d1d/9956238/2e3fdd52bada/genes-14-00290-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d1d/9956238/f22efdccde9d/genes-14-00290-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d1d/9956238/b1e749290bde/genes-14-00290-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d1d/9956238/149e5ed133c5/genes-14-00290-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d1d/9956238/b1560f669019/genes-14-00290-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d1d/9956238/1d7bce6d2b78/genes-14-00290-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d1d/9956238/ce392b11e3fc/genes-14-00290-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d1d/9956238/fc8c699bccbe/genes-14-00290-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d1d/9956238/2e3fdd52bada/genes-14-00290-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d1d/9956238/f22efdccde9d/genes-14-00290-g008.jpg

相似文献

1
DNA Double-Strand Break-Related Competitive Endogenous RNA Network of Noncoding RNA in Bovine Cumulus Cells.牛卵丘细胞中与 DNA 双链断裂相关的非编码 RNA 竞争性内源性 RNA 网络。
Genes (Basel). 2023 Jan 22;14(2):290. doi: 10.3390/genes14020290.
2
Integrated analysis of the expression profiles of the lncRNA-miRNA-mRNA ceRNA network in granulosa and cumulus cells from yak ovaries.牦牛卵巢颗粒细胞和卵丘细胞中 lncRNA-miRNA-mRNA ceRNA 网络表达谱的综合分析。
BMC Genomics. 2022 Sep 3;23(1):633. doi: 10.1186/s12864-022-08848-3.
3
Down-regulation of long non-coding RNAs in reproductive aging and analysis of the lncRNA-miRNA-mRNA networks in human cumulus cells.生殖衰老过程中长非编码 RNA 的下调及人卵丘细胞中 lncRNA-miRNA-mRNA 网络的分析。
J Assist Reprod Genet. 2022 Apr;39(4):919-931. doi: 10.1007/s10815-022-02446-8. Epub 2022 Mar 5.
4
Integrated analysis of microRNAs, circular RNAs, long non-coding RNAs, and mRNAs revealed competing endogenous RNA networks involved in brown adipose tissue whitening in rabbits.环状 RNA、长链非编码 RNA 和 mRNAs 的综合分析揭示了涉及兔棕色脂肪组织白化的竞争性内源性 RNA 网络。
BMC Genomics. 2022 Nov 28;23(1):779. doi: 10.1186/s12864-022-09025-2.
5
Comprehensive analysis of the whole coding and non-coding RNA transcriptome expression profiles and construction of the circRNA-lncRNA co-regulated ceRNA network in laryngeal squamous cell carcinoma.喉鳞状细胞癌全编码和非编码RNA转录组表达谱的综合分析及环状RNA-长链非编码RNA共调控的竞争性内源RNA网络构建
Funct Integr Genomics. 2019 Jan;19(1):109-121. doi: 10.1007/s10142-018-0631-y. Epub 2018 Aug 21.
6
Comprehensive identification of RNA transcripts and construction of RNA network in chronic obstructive pulmonary disease.慢性阻塞性肺疾病中 RNA 转录本的综合鉴定和 RNA 网络的构建。
Respir Res. 2022 Jun 11;23(1):154. doi: 10.1186/s12931-022-02069-8.
7
Whole transcriptome analysis of HCT-8 cells infected by Cryptosporidium parvum.微小隐孢子虫感染 HCT-8 细胞的全转录组分析。
Parasit Vectors. 2022 Nov 24;15(1):441. doi: 10.1186/s13071-022-05565-4.
8
Identification of circRNA-lncRNA-miRNA-mRNA Competitive Endogenous RNA Network as Novel Prognostic Markers for Acute Myeloid Leukemia.环状 RNA-lncRNA-miRNA-mRNA 竞争性内源性 RNA 网络作为急性髓系白血病新型预后标志物的鉴定。
Genes (Basel). 2020 Jul 31;11(8):868. doi: 10.3390/genes11080868.
9
Construction and functional enrichment analysis of the competitive endogenous RNA regulatory network for nonarteritic anterior ischemic optic neuropathy based on high-throughput sequencing.基于高通量测序的非动脉炎性前部缺血性视神经病变竞争性内源性RNA调控网络的构建及功能富集分析
Funct Integr Genomics. 2022 Dec;22(6):1253-1267. doi: 10.1007/s10142-022-00914-z. Epub 2022 Nov 10.
10
mRNA, lncRNA and Circular RNA Expression Profiles in Granulosa Cells of Infertile Women with Ovarian Endometriosis.卵巢子宫内膜异位症不孕女性颗粒细胞中的mRNA、lncRNA和环状RNA表达谱
Reprod Sci. 2022 Oct;29(10):2937-2946. doi: 10.1007/s43032-022-00966-3. Epub 2022 Jul 7.

引用本文的文献

1
Mitochondrial dysfunction in oocytes: implications for fertility and ageing.卵母细胞中的线粒体功能障碍：对生育能力和衰老的影响。
J Ovarian Res. 2025 Aug 14;18(1):186. doi: 10.1186/s13048-025-01764-6.
2
miR-302d Targeting of CDKN1A Regulates DNA Damage and Steroid Hormone Secretion in Bovine Cumulus Cells.miR-302d 通过靶向 CDKN1A 调控牛卵丘细胞的 DNA 损伤和甾体激素分泌。
Genes (Basel). 2023 Dec 10;14(12):2195. doi: 10.3390/genes14122195.
3
Knockdown of KDM5B Leads to DNA Damage and Cell Cycle Arrest in Granulosa Cells via MTF1.

本文引用的文献

1
DNMT3A-mediated high expression of circ_0057504 promotes benzo[a]pyrene-induced DNA damage via the NONO-SFPQ complex in human bronchial epithelial cells.DNMT3A 介导的 circ_0057504 高表达通过 NONO-SFPQ 复合物促进人支气管上皮细胞中苯并[a]芘诱导的 DNA 损伤。
Environ Int. 2022 Dec;170:107627. doi: 10.1016/j.envint.2022.107627. Epub 2022 Nov 10.
2
Anti-cancer immune responses to DNA damage response inhibitors: Molecular mechanisms and progress toward clinical translation.针对DNA损伤反应抑制剂的抗癌免疫反应：分子机制及临床转化进展
Front Oncol. 2022 Oct 6;12:998388. doi: 10.3389/fonc.2022.998388. eCollection 2022.
3
通过MTF1敲低KDM5B可导致颗粒细胞中的DNA损伤和细胞周期停滞。
Curr Issues Mol Biol. 2023 Apr 7;45(4):3219-3237. doi: 10.3390/cimb45040210.
LncRNA OTUD6B-AS1 promotes paclitaxel resistance in triple negative breast cancer by regulation of miR-26a-5p/MTDH pathway-mediated autophagy and genomic instability.
长链非编码 RNA OTUD6B-AS1 通过调节 miR-26a-5p/MTDH 通路介导的自噬和基因组不稳定性促进三阴性乳腺癌对紫杉醇的耐药性。
Aging (Albany NY). 2021 Nov 5;13(21):24171-24191. doi: 10.18632/aging.203672.
4
Home and Away: The Role of Non-Coding RNA in Intracellular and Intercellular DNA Damage Response.《内外有别：非编码 RNA 在细胞内和细胞间 DNA 损伤反应中的作用》。
Genes (Basel). 2021 Sep 23;12(10):1475. doi: 10.3390/genes12101475.
5
Genetic insights into biological mechanisms governing human ovarian ageing.遗传视角下人类卵巢衰老的生物学机制。
Nature. 2021 Aug;596(7872):393-397. doi: 10.1038/s41586-021-03779-7. Epub 2021 Aug 4.
6
Oocyte specific lncRNA variant influences oocyte and embryo development.卵母细胞特异性长链非编码RNA变体影响卵母细胞和胚胎发育。
Noncoding RNA Res. 2021 Jun 26;6(2):107-113. doi: 10.1016/j.ncrna.2021.06.001. eCollection 2021 Jun.
7
Coping with DNA Double-Strand Breaks via ATM Signaling Pathway in Bovine Oocytes.通过 ATM 信号通路应对牛卵母细胞中的 DNA 双链断裂。
Int J Mol Sci. 2020 Nov 24;21(23):8892. doi: 10.3390/ijms21238892.
8
MiRNA-155 regulates cumulus cells function, oocyte maturation, and blastocyst formation.miRNA-155 调节卵丘细胞功能、卵母细胞成熟和囊胚形成。
Biol Reprod. 2020 Aug 21;103(3):548-559. doi: 10.1093/biolre/ioaa098.
9
Impact of DNA Damage Response and Repair (DDR) Gene Mutations on Efficacy of PD-(L)1 Immune Checkpoint Inhibition in Non-Small Cell Lung Cancer.DNA 损伤反应和修复（DDR）基因突变对非小细胞肺癌 PD-（L）1 免疫检查点抑制疗效的影响。
Clin Cancer Res. 2020 Aug 1;26(15):4135-4142. doi: 10.1158/1078-0432.CCR-19-3529. Epub 2020 Apr 24.
10
Oocyte Elimination Through DNA Damage Signaling from CHK1/CHK2 to p53 and p63.通过 CHK1/CHK2 到 p53 和 p63 的 DNA 损伤信号消除卵母细胞。
Genetics. 2020 Jun;215(2):373-378. doi: 10.1534/genetics.120.303182. Epub 2020 Apr 9.