Connexins 37、40 和 45、Pannexin 1 和波形蛋白在喉鳞状细胞癌中的表达。

Expression of Connexins 37, 40 and 45, Pannexin 1 and Vimentin in Laryngeal Squamous Cell Carcinomas.

机构信息

Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital of Split, Spinčićeva 1, 21000 Split, Croatia.

Department of Nephrology and Hemodialysis, University Hospital of Split, Šoltanska 1, 21000 Split, Croatia.

出版信息

Genes (Basel). 2023 Feb 9;14(2):446. doi: 10.3390/genes14020446.

DOI:10.3390/genes14020446

PMID:36833374

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9956287/

Abstract

UNLABELLED

Approximately 60% of patients with squamous cell carcinoma (LSCC) have regional occult metastatic disease/distant metastases at the time of diagnosis, putting them at higher risk for disease progression. Therefore, biomarkers are needed for early prognostic purpose. The aim of this study was to analyze the expression pattern of connexins (Cx) 37, 40 and 45, pannexin1 (Panx1) and vimentin in LSCC and correlate with tumor grade (G) and outcome.

METHODS

Thirty-four patients who underwent (hemi-)laryngectomy and regional lymphadenectomy due to LSCC from 2017 to 2018 in University Hospital Split, Croatia, were studied. Samples of tumor tissue and adjacent normal mucosa embedded in paraffin blocks were stained using the immunofluorescence method and were semi-quantitatively analyzed.

RESULTS

The expression of Cx37, Cx40, and Panx1 differed between cancer and adjacent normal mucosa and between histological grades, being the highest in well-differentiated (G1) cancer and low/absent in poorly differentiated (G3) cancer (all < 0.05). The expression of vimentin was the highest in G3 cancer. Expression of Cx45 was generally weak/absent, with no significant difference between cancer and the controls or between grades. Lower Panx1 and higher vimentin expression were found to be prognostic factors for regional metastatic disease. Lower Cx37 and 40 expressions were present in patients with disease recurrence after the three-year follow-up period.

CONCLUSION

Cx37 and Cx40, Panx1, and vimentin have the potential to be used as prognostic biomarkers for LSCC.

摘要

目的

分析连接蛋白（Cx）37、40 和 45、pannexin1（Panx1）和波形蛋白在鳞状细胞癌（LSCC）中的表达模式，并与肿瘤分级（G）和预后相关联。

方法

对 2017 年至 2018 年期间在克罗地亚斯普利特大学医院因 LSCC 接受（半）喉切除术和区域淋巴结切除术的 34 例患者进行了研究。使用免疫荧光法对石蜡包埋的肿瘤组织和相邻正常黏膜样本进行染色，并进行半定量分析。

结果

Cx37、Cx40 和 Panx1 的表达在癌症与相邻正常黏膜之间以及组织学分级之间存在差异，在分化良好（G1）的癌症中最高，在分化差（G3）的癌症中最低/不存在（均<0.05）。波形蛋白在 G3 癌症中的表达最高。Cx45 的表达通常较弱/不存在，癌症与对照组之间或分级之间均无显著差异。较低的 Panx1 和较高的波形蛋白表达被发现是区域转移疾病的预后因素。在三年随访期间，疾病复发的患者 Cx37 和 Cx40 的表达降低。