Department of Legal Medicine, Nihon University School of Medicine, Tokyo 1738610, Japan.
Department of Interdisciplinary Research for Medicine and Photonics, Institute of Post-LED, Tokushima 7700814, Japan.
Genes (Basel). 2023 Feb 12;14(2):471. doi: 10.3390/genes14020471.
Coronary artery disease (CAD) is a common and fatal cardiovascular disease. Among known CAD risk factors, miRNA polymorphisms, such as Has-miR-143 () and Has-miR-146a (, have emerged as important genetic markers of CAD. Despite many genetic association studies in multiple populations, no study assessing the association between CAD risk and SNPs of miR-143 and miR-146 was documented in the Japanese people. Therefore, using the TaqMan SNP assay, we investigated two SNP genotypes in 151 subjects with forensic autopsy-proven CAD. After pathological observation, we used ImageJ software to assess the degree of coronary artery atresia. Moreover, the genotypes and miRNA content of the two groups of samples with atresia <10% and >10% were analyzed. The results showed that the CC genotype of was more frequent in patients with CAD than in controls, which was associated with the risk of CAD in the study population. However, Has-miR-143 genotype did not show a clear correlation with the risk of CAD.
冠状动脉疾病（CAD）是一种常见且致命的心血管疾病。在已知的 CAD 风险因素中，miRNA 多态性，如 Has-miR-143（）和 Has-miR-146a（，已成为 CAD 的重要遗传标志物。尽管在多个人群中进行了许多遗传关联研究，但在日本人中没有评估 miR-143 和 miR-146 的 SNP 与 CAD 风险之间关联的研究。因此，我们使用 TaqMan SNP 检测试剂盒，在 151 名经法医尸检证实患有 CAD 的患者中检测了两个 SNP 基因型。在病理观察后，我们使用 ImageJ 软件评估了冠状动脉闭锁的程度。此外，我们分析了两组闭塞程度 <10%和 >10%的样本的基因型和 miRNA 含量。结果表明，与对照组相比，CAD 患者中 存在更多的 CC 基因型，这与研究人群中 CAD 的发病风险相关。然而，Has-miR-143 基因型与 CAD 的发病风险之间没有明显的相关性。
