Sugita K, Suzuki N, Nakajima H
Department of Pediatrics, Chiba University School of Medicine, Japan.
Mutat Res. 1987 Dec;192(4):271-6. doi: 10.1016/0165-7992(87)90068-6.
The alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) enhanced the levels of plasminogen activator (PA) activity in fibroblast cells derived from the skin of patients with tuberous sclerosis. The enhanced enzyme levels were not correlated with those of cloning efficiency nor those of DNA synthesis after MNNG treatment. Enzyme enhancement was also observed in fibroblasts of ataxia telangiectasia and in human neoplastic glia cells, but not in fibroblasts of normal children. The PA induction test may be sufficiently sensitive for the detection of the cellular defects of tuberous sclerosis.
烷基化剂N-甲基-N'-硝基-N-亚硝基胍（MNNG）可提高结节性硬化症患者皮肤来源的成纤维细胞中的纤溶酶原激活物（PA）活性水平。MNNG处理后，酶水平的升高与克隆效率及DNA合成水平均无相关性。在共济失调毛细血管扩张症患者的成纤维细胞以及人肿瘤性神经胶质细胞中也观察到了酶活性增强的现象，但在正常儿童的成纤维细胞中未观察到。PA诱导试验对于检测结节性硬化症的细胞缺陷可能具有足够的敏感性。
