Gonda M A, Braun M J, Carter S G, Kost T A, Bess J W, Arthur L O, Van der Maaten M J
Laboratory of Cell and Molecular Structure, National Cancer Institute-Frederick, Maryland 21701.
Nature. 1987;330(6146):388-91. doi: 10.1038/330388a0.
An infectious virus which causes persistent lymphocytosis, lymphadenopathy, lesions in the central nervous system (CNS), progressive weakness and emaciation was previously isolated from the leukocytes of cattle. Our present studies show that this virus encodes a reverse transcriptase (RT) with Mg2+ cation preference, replicates and induces syncytia in a variety of embryonic bovine tissues in vitro, and has a morphology most similar to the human immunodeficiency virus (HIV). Moreover, serologic analyses have demonstrated a conservation of epitopes between the major core protein of this bovine retrovirus and HIV. Shared antigenic determinants were also observed with other pathogenic retroviruses of the lentivirus subfamily. To resolve the phylogenetic relationship of this virus, proviral molecular clones were derived and used to determine the nucleotide sequence of the highly conserved RT domain. The sequence data and serologic analyses together show that this bovine retrovirus is a novel lentivirus related to HIV and other lentiviruses. We propose that this virus be tentatively named bovine immunodeficiency-like virus (BIV) to reflect its genetic relationship and biological similarity to HIV.
一种可导致持续性淋巴细胞增多、淋巴结病、中枢神经系统（CNS）病变、进行性虚弱和消瘦的传染性病毒，此前已从牛的白细胞中分离出来。我们目前的研究表明，这种病毒编码一种偏好Mg2+阳离子的逆转录酶（RT），在体外能在多种牛胚胎组织中复制并诱导形成多核巨细胞，其形态与人类免疫缺陷病毒（HIV）最为相似。此外，血清学分析表明，这种牛逆转录病毒的主要核心蛋白与HIV之间存在表位保守性。在慢病毒亚科的其他致病性逆转录病毒中也观察到了共同的抗原决定簇。为了确定这种病毒的系统发育关系，我们获得了前病毒分子克隆，并用于确定高度保守的RT结构域的核苷酸序列。序列数据和血清学分析共同表明，这种牛逆转录病毒是一种与HIV和其他慢病毒相关的新型慢病毒。我们建议将这种病毒暂时命名为牛免疫缺陷样病毒（BIV），以反映其与HIV的遗传关系和生物学相似性。
