Jiang Dabao, Lu Xiaoyu, Zhang Ling, Tang Fang
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.
College of Forestry, Nanjing Forestry University, Nanjing 210037, China.
Insects. 2023 Jan 30;14(2):140. doi: 10.3390/insects14020140.
Snyder is a serious pest in China, and the odorant receptor co-receptor gene plays a crucial role in olfaction. However, the function of in the resistance of termites to entomopathogens has not been reported. We constructed ds-HT115 engineered bacteria based on the sequence from the full-length transcriptome data of . The engineered bacteria expressed dsRNA of . Sonication was used to inactivate the dsRNA-HT115 strain and obtain a large amount of ds. The ds produced using this method overcame the problem that genetically engineered bacteria could not be applied directly and improved its effectiveness against termites. Bioassays using the ds generated using this method showed that ds significantly increased the toxicity of the bacterial and fungal pathogens to . The present study showed, for the first time, the function of in termite resistance to pathogens, and the results provide a theoretical basis for the development and application of termite RNA biopesticides.
斯奈德白蚁在中国是一种严重的害虫，气味受体共受体基因在嗅觉中起着关键作用。然而，该基因在白蚁对昆虫病原体抗性方面的功能尚未见报道。我们基于斯奈德白蚁全长转录组数据中的该基因序列构建了ds-HT115工程菌。该工程菌表达了该基因的dsRNA。采用超声处理使dsRNA-HT115菌株失活并获得大量dsRNA。用这种方法产生的dsRNA克服了基因工程菌不能直接应用的问题，并提高了其对白蚁的防治效果。使用该方法产生的dsRNA进行的生物测定表明，dsRNA显著提高了细菌和真菌病原体对斯奈德白蚁的毒性。本研究首次揭示了该基因在白蚁对病原体抗性中的功能，研究结果为白蚁RNA生物农药的开发和应用提供了理论依据。
