Patrinou Vasiliki, Patsialou Stefania, Daskalaki Alexandra, Economou Christina N, Aggelis George, Vayenas Dimitris V, Tekerlekopoulou Athanasia G
Department of Sustainable Agriculture, University of Patras, 30100 Agrinio, Greece.
Department of Chemical Engineering, School of Engineering, University of Patras, 26500 Patras, Greece.
Life (Basel). 2023 Feb 9;13(2):480. doi: 10.3390/life13020480.
Marine microalgae are considered an important feedstock of multiple valuable metabolic compounds of high biotechnological potential. In this work, the marine microalga was cultivated in different scaled photobioreactors (PBRs). Initially, experiments were performed using two different growth substrates (a modified F/2 and the commercial fertilizer Nutri-Leaf (30% TN-10% P-10% K)) to identify the most efficient and low-cost growth medium. These experiments took place in 4 L glass aquariums at the laboratory scale and in a 9 L vertical tubular pilot column. Enhanced biomass productivities (up to 83.2 mg L d) and improved biomass composition (up to 41.8% d.w. proteins, 18.7% d.w. carbohydrates, 25.7% d.w. lipids and 4.2% d.w. total chlorophylls) were found when the fertilizer was used. Pilot-scale experiments were then performed using Nutri-Leaf as a growth medium in different PBRs: (a) a paddle wheel, open, raceway pond of 40 L, and (b) a disposable polyethylene (plastic) bag of 280 L working volume. Biomass growth and composition were also monitored at the pilot scale, showing that high-quality biomass can be produced, with important lipids (up to 27.6% d.w.), protein (up to 45.3% d.w.), carbohydrate (up to 15.5% d.w.) and pigment contents (up to 4.2% d.w. total chlorophylls), and high percentages of eicosapentaenoic acid (EPA). The research revealed that the strain successfully escalated in larger volumes and the biochemical composition of its biomass presents high commercial interest and could potentially be used as a feed ingredient.
海洋微藻被认为是多种具有高生物技术潜力的有价值代谢化合物的重要原料。在这项工作中，该海洋微藻在不同规模的光生物反应器（PBR）中进行培养。最初，使用两种不同的生长底物（改良的F/2和商业肥料Nutri-Leaf（30%总氮-10%磷-10%钾））进行实验，以确定最有效和低成本的生长培养基。这些实验在实验室规模的4升玻璃水族箱和9升垂直管式中试柱中进行。当使用该肥料时，发现生物量生产力提高（高达83.2毫克/升·天），生物量组成改善（高达41.8%干重蛋白质、18.7%干重碳水化合物、25.7%干重脂质和4.2%干重总叶绿素）。然后在不同的PBR中使用Nutri-Leaf作为生长培养基进行中试规模实验：（a）一个40升的桨轮式开放式跑道池塘，和（b）一个工作体积为280升的一次性聚乙烯（塑料）袋。在中试规模下也监测了生物量的生长和组成，结果表明可以生产出高质量的生物量，其具有重要的脂质（高达27.6%干重）、蛋白质（高达45.3%干重）、碳水化合物（高达15.5%干重）和色素含量（高达4.2%干重总叶绿素），以及高比例的二十碳五烯酸（EPA）。该研究表明，该菌株在更大体积中成功扩繁，其生物量的生化组成具有很高的商业价值，有可能用作饲料成分。
