Needels D L, Nieto-Sampedro M, Cotman C W
Department of Psychobiology, University of California, Irvine 92717.
Neurochem Res. 1987 Oct;12(10):901-7. doi: 10.1007/BF00966312.
Ciliary ganglion neurons and nonneurons can be separated from each other, based on the greater adhesivity of the nonneurons to untreated tissue culture plastic in the presence of serum. When the separation was carried out in the presence of Serum Plus (a commercially available supplemented serum), two populations of neurons were distinguished. Neurons in the first class (50-60% of total) adhered to plastic within 15 min, tended to aggregate into clumps, and were not well supported in long term culture by brain extracts. Neuronal adhesion to plastic was inhibited by heparin but not by chondroitin sulfate. Neurons in the second class did not attach to plastic for up to 90 min (and could thus be purified), were not as prone to aggregation, and were quantitatively supported for long periods (greater than 2 weeks) by the neurotrophic factor(s) present in extracts of injured brain. Although no direct evidence is provided, these populations may correspond to the well characterized ciliary and choroid neurons.
