Department of Gynecology, Maternal and Child Medical Centre of Anhui Medical University, Hefei 230001, China.
Department of Gynecology, Anhui Province Maternity and Child Healthcare Hospital, Hefei 230001, China.
Medicina (Kaunas). 2023 Feb 13;59(2):358. doi: 10.3390/medicina59020358.
: Cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) are malignant disorders with adverse prognoses for advanced patients. Anoikis, which is involved in tumor metastasis, facilitates the survival and separation of tumor cells from their initial site. Unfortunately, it is rarely studied, and in the literature, studies have only addressed the prognosis character of anoikis for patients with CESC. : We utilized anoikis-related genes (ANRGs) to construct a prognostic signature in CESC patients that were selected from the Genecards and Harmonizome portals. Furthermore, we revealed the underlying clinical value of this signature for clinical maneuvers by providing clinical specialists with an innovative nomogram on the basis of ANRGs. Finally, we investigated the immune microenvironment and drug sensitivity in different risk groups. : We screened six genes from fifty-eight anoikis-related differentially expressed genes in the TCGA-CESC cohort, and we constructed a prognostic signature. Then, we built a nomogram combined with CESC clinicopathological traits and risk scores, which demonstrated that this model may improve the prognosis of CESC patients in clinical therapy. Next, the prognostic risk scores were confirmed to be an independent prognostic indicator. Additionally, we programmed a series of analyses, which included immune infiltration analysis, therapy-related analysis, and GSVA enrichment analysis, to identify the functions and mechanisms of the prognostic models during the progression of cancer in CESC patients. Finally, we performed quantitative reverse transcription polymerase chain reaction (qRT-PCR) to verify the six ANRGs. : The present discovery verified that the predictive 6-anoikis-related gene (6-ANRG) signature and nomogram serve as imperative factors that might notably impact a CESC patient's prognosis, and they may be able to provide new clinical evidence to assume the role of underlying biological biomarkers and thus become indispensable indicators for prospective diagnoses and advancing therapy.
宫颈鳞状细胞癌和宫颈内膜腺癌（CESC）是恶性疾病，晚期患者预后不良。细胞凋亡，涉及肿瘤转移，促进肿瘤细胞与其初始部位的分离和存活。不幸的是，它很少被研究，在文献中，研究仅针对 CESC 患者的细胞凋亡预后特征。
我们利用与细胞凋亡相关的基因（ANRGs）构建了一个 CESC 患者的预后模型，该模型是从 Genecards 和 Harmonizome 门户中选择的。此外，我们根据 ANRGs 为临床专家提供创新的列线图，揭示了该模型对临床操作的潜在临床价值。最后，我们研究了不同风险组的免疫微环境和药物敏感性。
我们从 TCGA-CESC 队列的 58 个与细胞凋亡相关的差异表达基因中筛选出六个基因，并构建了一个预后模型。然后，我们构建了一个结合 CESC 临床病理特征和风险评分的列线图，表明该模型可能改善 CESC 患者的临床治疗预后。接下来，预后风险评分被确认为独立的预后指标。此外，我们还进行了一系列分析，包括免疫浸润分析、治疗相关分析和 GSVA 富集分析，以确定预后模型在 CESC 患者癌症进展过程中的功能和机制。最后，我们进行了定量逆转录聚合酶链反应（qRT-PCR）验证六个 ANRGs。
本研究验证了预测性 6-与细胞凋亡相关基因（6-ANRG）标志和列线图是影响 CESC 患者预后的重要因素，它们可能为 CESC 患者提供新的临床证据，假设其作为潜在生物标志物的作用，并成为前瞻性诊断和治疗进展的不可或缺指标。
