Giufrè Maria, Cardines Rita, Marra Manuela, Carollo Maria, Cerquetti Marina, Stefanelli Paola
Department of Infectious Diseases, Istituto Superiore di Sanità, 00161 Rome, Italy.
Core Facilities Technical-Scientific Service (FAST), Istituto Superiore di Sanità, 00161 Rome, Italy.
Microorganisms. 2023 Jan 26;11(2):315. doi: 10.3390/microorganisms11020315.
invasive disease is a severe infection that needs rapid antibiotic therapy. The aim of the study was to perform and evaluate the serotype distribution, antibiotic susceptibility and molecular characteristics of 392 invasive isolates collected during 2017-2021 in Italy. The majority of isolates were NTHi (305/392, 77.8%), followed by Hib (49/392, 12.5%). Ampicillin resistance was frequently detected (85/392, 21.7%): 12.2% were β-lactamase producers (all except one ), 9.4% were β-lactamase-negative ampicillin-resistant (BLNAR), with mutations in the I gene. Six isolates were resistant to ciprofloxacin, with substitutions in GyrA and ParC. An MLST analysis revealed the occurrence of international resistant clones, such as ST103 and ST14, highlighting the importance of molecular surveillance.
侵袭性疾病是一种需要快速抗生素治疗的严重感染。本研究的目的是对2017年至2021年期间在意大利收集的392株侵袭性分离株进行血清型分布、抗生素敏感性及分子特征分析与评估。大多数分离株为非产β-内酰胺酶的流感嗜血杆菌（NTHi，305/392，77.8%），其次是b型流感嗜血杆菌（Hib，49/392，12.5%）。经常检测到氨苄西林耐药（85/392，21.7%）：12.2%为β-内酰胺酶产生菌（除1株外均为），9.4%为β-内酰胺酶阴性氨苄西林耐药（BLNAR），I基因存在突变。6株分离株对环丙沙星耐药，GyrA和ParC发生替换。多位点序列分型（MLST）分析显示存在国际耐药克隆，如ST103和ST14，突出了分子监测的重要性。
