Molecular Physical-Chemistry R&D Unit, Department of Chemistry, University of Coimbra, 3004-535 Coimbra, Portugal.
Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal.
Molecules. 2023 Feb 10;28(4):1698. doi: 10.3390/molecules28041698.
A dinuclear Pt(II) complex with putrescine as bridging polyamine ligand ([PtPut(NH)]Cl) was synthesized and assessed as to its potential anticancer activity against a human non-small cell lung cancer line (A549), as well as towards non-cancer cells (BEAS-2B). This effect was evaluated through in vitro cytotoxicity assays (MTT and SRB) coupled to microFTIR and microRaman spectroscopies, the former delivering information on growth-inhibiting and cytotoxic abilities while the latter provided very specific information on the metabolic impact of the metal agent (at the sub-cellular level). Regarding cancer cells, a major impact of [PtPut(NH)]Cl was evidenced on cellular proteins and lipids, as compared to DNA, particularly via the Amide I and Amide II signals. The effect of the chelate on non-malignant cells was lower than on malignant ones, evidencing a promising low toxicity towards healthy cells.
合成了一种具有腐胺作为桥联多胺配体的双核 Pt(II) 配合物([PtPut(NH)]Cl)，并评估了其对人非小细胞肺癌细胞系(A549)以及非癌细胞(BEAS-2B)的潜在抗癌活性。通过体外细胞毒性测定(MTT 和 SRB)与微傅里叶变换红外光谱(microFTIR)和微拉曼光谱(microRaman spectroscopy)相结合来评估这种效果，前者提供关于生长抑制和细胞毒性能力的信息，而后者提供关于金属试剂代谢影响的非常具体的信息(在亚细胞水平上)。对于癌细胞，与 DNA 相比，[PtPut(NH)]Cl 对细胞蛋白和脂质有更大的影响，特别是通过酰胺 I 和酰胺 II 信号。螯合物对非恶性细胞的作用低于对恶性细胞的作用，这表明对健康细胞具有有希望的低毒性。
