Awad Muhammed, Kopecki Zlatko, Barnes Timothy J, Wignall Anthony, Joyce Paul, Thomas Nicky, Prestidge Clive A
Centre for Pharmaceutical Innovation, University of South Australia, Clinical and Health Sciences, Adelaide, SA 5000, Australia.
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, Assiut 71524, Egypt.
Pharmaceutics. 2023 Jan 17;15(2):305. doi: 10.3390/pharmaceutics15020305.
Cutaneous chronic wounds impose a silent pandemic that affects the lives of millions worldwide. The delayed healing process is usually complicated by opportunistic bacteria that infect wounds. is one of the most prevalent bacteria in infected cutaneous wounds, with the ability to form antibiotic-resistant biofilms. Recently, we have demonstrated the potential of gallium protoporphyrin lipid liquid crystalline nanoparticles (GaPP-LCNP) as a photosensitizer against biofilms in vitro. Herein, we investigate the potential of GaPP-LCNP using a pre-clinical model of infected cutaneous wounds. GaPP-LCNP showed superior antibacterial activity compared to unformulated GaPP, reducing biofilm bacterial viability by 5.5 log compared to 2.5 log in an ex vivo model, and reducing bacterial viability by 1 log in vivo, while unformulated GaPP failed to reduce bacterial burden. Furthermore, GaPP-LCNP significantly promoted wound healing through reduction in the bacterial burden and improved early collagen deposition. These findings pave the way for future pre-clinical investigation and treatment optimizations to translate GaPP-LCNP towards clinical application.
皮肤慢性伤口构成了一场无声的大流行，影响着全球数百万人的生活。延迟的愈合过程通常因感染伤口的机会性细菌而变得复杂。[细菌名称]是感染性皮肤伤口中最常见的细菌之一，具有形成抗生素抗性生物膜的能力。最近，我们已经证明了镓原卟啉脂质液晶纳米颗粒（GaPP-LCNP）作为一种针对[细菌名称]生物膜的体外光敏剂的潜力。在此，我们使用感染性皮肤伤口的临床前模型研究了GaPP-LCNP的潜力。与未配制的GaPP相比，GaPP-LCNP显示出优异的抗菌活性，在体外模型中，与未配制的GaPP使生物膜细菌活力降低2.5个对数相比，GaPP-LCNP使生物膜细菌活力降低5.5个对数，在体内使细菌活力降低1个对数，而未配制的GaPP未能降低细菌负荷。此外，GaPP-LCNP通过减少细菌负荷和改善早期胶原蛋白沉积，显著促进了伤口愈合。这些发现为未来的临床前研究和治疗优化铺平了道路，以便将GaPP-LCNP转化为临床应用。
