Guo Gang, Xu Shuhao, Chen Hao, Hao Yuanfeng, Mao Hailiang
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Plants (Basel). 2023 Feb 8;12(4):759. doi: 10.3390/plants12040759.
Pre-harvest sprouting (PHS) of wheat reduces grain yield and quality, and it is strongly affected by seed dormancy. Therefore, identification of quantitative trait loci (QTL) for seed dormancy is essential for PHS resistance breeding. A doubled haploid (DH) population, consisting of 174 lines from the cross between Yangmai16 (YM16) and Zhongmai895 (ZM895) was used to detect QTLs for seed dormancy and grain color. For seed dormancy, a total of seven QTLs were detected on chromosomes 2A, 3A, 3D, 4D, 5B and 5D over four environments, among which , and were stably detected in more than two environments. For grain color, only two QTLs, and were detected on chromosomes 3A and 3D, which physically overlapped with and , respectively. has never been reported elsewhere and is probably a novel locus with allelic effect of seed dormancy contributed by weakly dormant parent ZM895, and a KASP marker was developed and validated in a wheat natural population. This study provides new information on the genetic dissection of seed dormancy, which may aid in further improvement for marker-assisted wheat breeding for PHS resistance.
小麦收获前穗发芽（PHS）会降低籽粒产量和品质，且其受种子休眠的影响很大。因此，鉴定种子休眠的数量性状位点（QTL）对于抗穗发芽育种至关重要。利用由扬麦16（YM16）和中麦895（ZM895）杂交产生的174个株系组成的加倍单倍体（DH）群体，检测种子休眠和籽粒颜色的QTL。对于种子休眠，在四个环境中，于2A、3A、3D、4D、5B和5D染色体上共检测到7个QTL，其中， 、 和 在两个以上环境中被稳定检测到。对于籽粒颜色，仅在3A和3D染色体上检测到两个QTL， 和 ，它们分别与 和 物理重叠。 在其他地方从未被报道过，可能是一个由弱休眠亲本ZM895贡献的具有种子休眠等位基因效应的新位点，并开发了一个KASP标记并在小麦自然群体中进行了验证。本研究为种子休眠的遗传剖析提供了新信息，这可能有助于进一步改进小麦抗穗发芽的分子标记辅助育种。
