Maldonado Yessenia E, Malagón Omar, Cumbicus Nixon, Gilardoni Gianluca
Departamento de Química, Universidad Técnica Particular de Loja (UTPL), Calle Marcelino Champagnat s/n, Loja 110107, Ecuador.
Departamento de Ciencias Biológicas y Agropecuarias, Universidad Técnica Particular de Loja (UTPL), Calle Marcelino Champagnat s/n, Loja 110107, Ecuador.
Plants (Basel). 2023 Feb 14;12(4):849. doi: 10.3390/plants12040849.
An essential oil, distilled from the leaves of the Andean species Muschl., is described in the present study for the first time. The chemical composition was qualitatively and quantitatively determined by GC-MS and GC-FID, respectively. On the one hand, the qualitative composition was obtained by comparing the mass spectrum and the linear retention index of each component with data from literature. On the other hand, the quantitative composition was determined by calculating the relative response factor of each constituent, according to its combustion enthalpy. Both analyses were carried out with two orthogonal columns of nonpolar and polar stationary phases. A total of 112 compounds were detected and quantified with at least one column, corresponding to 87.3-93.0% of the whole oil mass. Among the 112 detected components, 103 were identified. The main constituents were α-pinene (5.3-6.0%), ()-β-caryophyllene (2.4-2.8%), α-humulene (3.0-3.2%), germacrene D (4.9-6.5%), δ-cadinene (2.2-2.3%), caryophyllene oxide (1.6-2.2%), α-cadinol (3.8-4.4%), 1-nonadecanol (1.7-1.9%), 1-eicosanol (0.9-1.2%), -tricosane (3.3-3.4%), 1-heneicosanol (4.5-5.8%), -pentacosane (5.8-7.1%), 1-tricosanol (4.0-4.5%), and -heptacosane (3.0-3.5%). Furthermore, an enantioselective analysis was carried out on the essential oil, by means of two cyclodextrin-based capillary columns. The enantiomers of α-pinene, β-pinene, sabinene, α-phellandrene, β-phellandrene, linalool, α-copaene, terpinen-4-ol, α-terpineol, and germacrene D were detected, and the respective enantiomeric excess was calculated.
本研究首次描述了一种从安第斯物种穆氏（Muschl.）的叶子中蒸馏得到的精油。其化学成分分别通过气相色谱 - 质谱联用仪（GC - MS）和气相色谱 - 氢火焰离子化检测器（GC - FID）进行定性和定量测定。一方面，通过将每种成分的质谱和线性保留指数与文献数据进行比较来获得定性组成。另一方面，根据各成分的燃烧焓计算相对响应因子来确定定量组成。这两种分析均使用了非极性和极性固定相的两根正交柱进行。总共检测并定量了112种化合物，至少在一根柱上有响应，占整个油质量的87.3 - 93.0%。在检测到的112种成分中，鉴定出了103种。主要成分包括α - 蒎烯（5.3 - 6.0%）、（） - β - 石竹烯（2.4 - 2.8%）、α - 葎草烯（3.0 - 3.2%）、杜松烯D（4.9 - 6.5%）、δ - 杜松烯（2.2 - 2.3%）、石竹烯氧化物（1.6 - 2.2%）、α - 杜松醇（3.8 - 4.4%）、1 - 十九烷醇（1.7 - 1.9%）、1 - 二十烷醇（0.9 - 1.2%）、二十三烷（3.3 - 3.4%）、1 - 二十一烷醇（4.5 - 5.8%）、二十五烷（5.8 - 7.1%）、1 - 二十三烷醇（4.0 - 4.5%）和二十七烷（3.0 - 3.5%）。此外，通过两根基于环糊精的毛细管柱对该精油进行了对映体选择性分析。检测到了α - 蒎烯、β - 蒎烯、桧烯、α - 水芹烯、β - 水芹烯、芳樟醇、α - 可巴烯、萜品醇 - 4、α - 松油醇和杜松烯D的对映体，并计算了各自的对映体过量值。
