Malagón Omar, Cartuche Patricio, Montaño Angel, Cumbicus Nixon, Gilardoni Gianluca
Departamento de Química, Universidad Técnica Particular de Loja (UTPL), Calle Marcelino Champagnat s/n, Loja 110107, Ecuador.
Departamento de Ciencias Biológicas, Universidad Técnica Particular de Loja (UTPL), Calle Marcelino Champagnat s/n, Loja 110107, Ecuador.
Plants (Basel). 2022 Jan 31;11(3):398. doi: 10.3390/plants11030398.
A previously uninvestigated essential oil (EO) was distilled from Cuatrec. (Asteraceae) and submitted to chemical and enantioselective analyses. The qualitative and quantitative analyses were conducted by GC-MS and GC-FID, over two orthogonal columns (5%-phenyl-methylpolysiloxane and polyethylene glycol stationary phases). Major constituents (≥2%) were, on both columns, respectively, as follows: α-phellandrene (16.1-17.2%), α-pinene (14.0-15.0%), germacrene D (13.3-14.8%), -myrtanol acetate (8.80%), δ-cadinene (4.2-4.6%), β-phellandrene (3.3-2.8%), ()-β-caryophyllene (3.1-2.0%), -cymene (2.4%), α-cadinol (2.3-2.6%), and α-humulene (1.7-2.0%). All the quantified compounds corresponded to 93.5-97.3% by weight of the whole essential oil, with monoterpenes counting for 53.8-55.6% of the total, and sesquiterpenes for 38.5-41.4%. For what concerns the enantioselective analyses, the chiral components were investigated through a β-cyclodextrin-based enantioselective column (2,3-diethyl-6--butyldimethylsilyl-β-cyclodextrin). A total of six chiral metabolites were analysed and the respective enantiomeric excess calculated as follows: (1,5)-(-)-α-pinene (98.2%), (1,5)-(-)-β-pinene (11.9%), (1,5)-(+)-sabinene (14.0%), ()-(-)-α-phellandrene (100.0%), ()-(-)-β-phellandrene (100.0%), and ()-(-)-germacrene D (95.5%). According to the chemical composition and enantiomeric distribution of major compounds, this EO can be considered promising as a cholinergic, antiviral and, probably, analgesic product.
从菊科植物Cuatrec.中提取了一种此前未被研究的精油（EO），并对其进行了化学和对映体选择性分析。定性和定量分析通过气相色谱 - 质谱联用仪（GC - MS）和气相色谱 - 氢火焰离子化检测器（GC - FID），在两根正交柱（5%-苯基 - 甲基聚硅氧烷和聚乙二醇固定相）上进行。在两根柱上，主要成分（≥2%）分别如下：α-水芹烯（16.1 - 17.2%）、α-蒎烯（14.0 - 15.0%）、吉马烯D（13.3 - 14.8%）、乙酸桃金娘醇酯（8.80%）、δ-杜松烯（4.2 - 4.6%）、β-水芹烯（3.3 - 2.8%）、（）-β-石竹烯（3.1 - 2.0%）、对异丙基甲苯（2.4%）、α-杜松醇（2.3 - 2.6%）和α-葎草烯（1.7 - 2.0%）。所有定量化合物占整个精油重量的93.5 - 97.3%，其中单萜类占总量的53.8 - 55.6%，倍半萜类占38.5 - 41.4%。关于对映体选择性分析，通过基于β-环糊精的对映体选择性柱（2,3 - 二乙基 - 6 - 叔丁基二甲基甲硅烷基 - β-环糊精）研究手性成分。总共分析了六种手性代谢物，其各自的对映体过量计算如下：（1,5）-（-）-α-蒎烯（98.2%）、（1,5）-（-）-β-蒎烯（11.9%）、（1,5）-（+）-桧烯（14.0%）、（）-（-）-α-水芹烯（100.0%）、（）-（-）-β-水芹烯（100.0%）和（）-（-）-吉马烯D（95.5%）。根据主要化合物的化学组成和对映体分布，这种精油有望成为一种胆碱能、抗病毒且可能具有镇痛作用的产品。
