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燕麦β-葡聚糖对鲢鱼鱼糜凝胶特性和蛋白质构象的影响

Effect of oat β-glucan on gel properties and protein conformation of silver carp surimi.

作者信息

He Xueli, Lv Yanan, Li Xuepeng, Yi Shumin, Zhao Honglei, Xu Yongxia, Li Jianrong

机构信息

College of Food Science and Engineering, Bohai University, National R&D Branch Center of Surimi and Surimi Products Processing, Jinzhou, P. R. China.

出版信息

J Sci Food Agric. 2023 May;103(7):3367-3375. doi: 10.1002/jsfa.12525. Epub 2023 Mar 8.

Abstract

BACKGROUND

Polysaccharides are the most widely used additives to enhance the quality of surimi gels. Oat β-glucan (OG), a functional polysaccharide, is known to affect the gelation characteristics of surimi. However, it has been rarely reported. Therefore, the effect of OG at different levels on gelling properties, protein conformation, and microstructures of silver carp surimi gels were investigated.

RESULTS

An increase in the OG content from 0 to 1.0% significantly improved the hardness, springiness, chewiness, puncture properties, storage modulus, and loss modulus of surimi gels. Moreover, the incorporation of OG (0-1.0%) facilitated the unfolding of proteins, resulting in the conformational transformation from α-helix to β-sheet and β-turn. Consequently, surimi-OG gels displayed a denser network structure with smaller and more uniform voids. Furthermore, partial free water in the gel network was converted into immobile water, increasing the water-holding capacity. However, a further increase in the OG concentration (1.0-2.0%) resulted in a looser and more uneven network structure with large and numerous cavities. In addition, the whiteness of composite gels decreased with increasing content of OG.

CONCLUSION

The addition of 1.0% OG dramatically improved the gelation performance of silver carp surimi, providing a theoretical foundation for the exploitation and manufacture of functional surimi products. © 2023 Society of Chemical Industry.

摘要

背景

多糖是用于提高鱼糜凝胶品质的最广泛使用的添加剂。燕麦β-葡聚糖(OG)作为一种功能性多糖,已知会影响鱼糜的凝胶化特性。然而,相关报道很少。因此,研究了不同水平的OG对鲢鱼鱼糜凝胶的凝胶特性、蛋白质构象和微观结构的影响。

结果

OG含量从0增加到1.0%显著提高了鱼糜凝胶的硬度、弹性、咀嚼性、穿刺性能、储能模量和损耗模量。此外,添加OG(0 - 1.0%)促进了蛋白质的展开,导致构象从α-螺旋转变为β-折叠和β-转角。因此,鱼糜 - OG凝胶呈现出更致密的网络结构,孔隙更小且更均匀。此外,凝胶网络中的部分自由水转化为结合水,提高了持水能力。然而,OG浓度进一步增加(1.0 - 2.0%)导致网络结构更松散、更不均匀,有大且多的空洞。此外,复合凝胶的白度随OG含量的增加而降低。

结论

添加1.0%的OG显著改善了鲢鱼鱼糜的凝胶化性能,为功能性鱼糜产品的开发和制造提供了理论基础。© 2023化学工业协会。

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