Laboratory of Enzymology, Department of Cell Biology, University of Brasília, Brasilia, DF, 70910-900, Brazil.
US DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
World J Microbiol Biotechnol. 2023 Feb 25;39(4):105. doi: 10.1007/s11274-023-03546-y.
The gram-positive bacterium Clostridium thermocellum contains a set of carbohydrate-active enzymes that can potentially be employed to generate high-value-added products from lignocellulose. In this study, the gene expression profiling of C. thermocellum B8 was provided during growth in the presence of sugarcane bagasse and straw as a carbon source in comparison to growth using microcrystalline cellulose. A total of 625 and 509 genes were up-regulated for growth in the presence of bagasse and straw, respectively. These genes were mainly grouped into carbohydrate-active enzymes (CAZymes), cell motility, chemotaxis, quorum sensing pathway and expression control of glycoside hydrolases. These results show that type of carbon source modulates the gene expression profiling of carbohydrate-active enzymes. In addition, highlight the importance of cell motility, attachment to the substrate and communication in deconstructing complex substrates. This present work may contribute to the development of enzymatic cocktails and industrial strains for biorefineries based on sugarcane residues as feedstock.
产热梭菌（Clostridium thermocellum）是一种革兰氏阳性菌，其细胞内存在一整套能将木质纤维素转化为高附加值产品的碳水化合物活性酶。在本研究中，我们对以甘蔗渣和秸秆为碳源时产热梭菌 B8 的基因表达情况进行了分析，并与以微晶纤维素为碳源时的生长情况进行了比较。结果显示，与微晶纤维素相比，在甘蔗渣和秸秆存在的情况下分别有 625 个和 509 个基因上调表达。这些基因主要被分为碳水化合物活性酶（CAZymes）、细胞运动性、趋化性、群体感应途径和糖苷水解酶的表达调控等几类。上述结果表明，碳源的类型会调节碳水化合物活性酶的基因表达谱。此外，该结果还强调了细胞运动性、与底物的附着以及细胞间通讯在复杂底物的解构过程中的重要性。本研究可为以甘蔗渣为原料的生物炼制厂开发酶制剂和工业菌株提供参考。
