Brebu Mihai, Simion Violeta Elena, Andronie Viorel, Jaimes-Mogollón Aylen Lisset, Beleño-Sáenz Kelvin de Jesús, Ionescu Florina, Welearegay Tesfalem Geremariam, Suschinel Raluca, de Lema Jose Bruno, Ionescu Radu
"Petru Poni" Institute of Macromolecular Chemistry, Aleea Grigore Ghica Voda 41A, 700487, Iasi, Romania.
Faculty of Veterinary Medicine, Spiru Haret University, Bd. Basarabia 256, 030171, Bucharest, Romania.
Mol Cell Biochem. 2023 Nov;478(11):2473-2480. doi: 10.1007/s11010-023-04676-5. Epub 2023 Feb 25.
Bovine tuberculosis (bTB) is an infectious disease with significant impact on animal health, public health and international trade. Standard bTB screening in live cattle consists in injecting tuberculin and measuring the swelling at the place of injection few days later. This procedure is expensive, time-consuming, logistically challenging, and is not conclusive before performing confirmatory tests and additional analysis. The analysis of the volatile organic compounds (VOCs) emitted by non-invasive biological samples can provide an alternative diagnostic approach suitable for bTB screening. In the present study, we analyzed VOC samples emitted through the breath, feces and skin of 18 cows diagnosed with bTB from three farms from Romania, as well as of 27 negative cows for bTB from the same farms. Analytical studies employing gas chromatography coupled to mass spectrometry revealed 80 VOCs emitted through the breath, 200 VOCs released by feces, and 80 VOCs emitted through the skin. Statistical analysis of these compounds allowed the identification of 3 tentative breath VOC biomarkers (acetone; 4-methyldecane; D-limonene), 9 tentative feces VOC biomarkers (toluene; [(1,1-dimethylethyl)thio]acetic acid; alpha-thujene; camphene; phenol; o-cymene; 3-(1,1-dimethylethyl)-2,2,4,4-tetramethyl-3-pentanol; 2,5-dimethylhexane-2,5-dihydroperoxide; 2,4-di-tert-butylphenol), and 3 tentative skin VOC biomarkers (ammonia; 1-methoxy-2-propanol; toluene). The possible pathway of these volatile biomarkers is discussed.
牛结核病（bTB）是一种对动物健康、公共卫生和国际贸易有重大影响的传染病。对活牛进行标准的牛结核病筛查，包括注射结核菌素，并在几天后测量注射部位的肿胀情况。该程序成本高昂、耗时、在后勤方面具有挑战性，并且在进行确诊测试和额外分析之前并不能得出结论。对非侵入性生物样本释放的挥发性有机化合物（VOCs）进行分析，可以提供一种适用于牛结核病筛查的替代诊断方法。在本研究中，我们分析了来自罗马尼亚三个农场的18头被诊断患有牛结核病的奶牛以及来自同一农场的27头牛结核病阴性奶牛通过呼吸、粪便和皮肤释放的VOC样本。采用气相色谱-质谱联用的分析研究表明，通过呼吸释放了80种VOCs，粪便释放了200种VOCs，皮肤释放了80种VOCs。对这些化合物的统计分析使得能够鉴定出3种初步的呼吸VOC生物标志物（丙酮；4-甲基癸烷；D-柠檬烯）、9种初步的粪便VOC生物标志物（甲苯；[(1,1-二甲基乙基)硫代]乙酸；α-侧柏烯；莰烯；苯酚；邻异丙基甲苯；3-(1,1-二甲基乙基)-2,2,4,4-四甲基-3-戊醇；2,5-二甲基己烷-2,5-二氢过氧化物；2,4-二叔丁基苯酚）以及3种初步的皮肤VOC生物标志物（氨；1-甲氧基-2-丙醇；甲苯）。本文讨论了这些挥发性生物标志物的可能途径。
